TRANSFORMATION OF SENSORY ORGAN IDENTITY BY ECTOPIC EXPRESSION OF CUT IN DROSOPHILA
TRANSFORMATION OF SENSORY ORGAN IDENTITY BY ECTOPIC EXPRESSION OF CUT IN DROSOPHILA
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DOI：
10.1101/gad.5.7.1124
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发表时间：
1991-07-01
影响因子：
10.5
通讯作者：
JAN, YN
中科院分区：
文献类型：
--
作者：
BLOCHLINGER, K;JAN, LY;JAN, YN
cut活性的丧失导致外周神经系统中神经特性的改变，使得外部感觉器官的神经元和支持细胞转化为内部弦音器官的神经元和支持细胞。cut编码一个大的核同源结构域蛋白（Cut），其在es器官及其前体的分化细胞中表达，但不在ch器官的细胞中表达。现在，我们分析异位切表达的影响，在果蝇的cut-编码序列的诱导调控下的cut-线。我们表明，无处不在的切表达在胚胎中的结果，特别是在形态和抗原转化的ch器官到es器官。这种效应似乎涉及切割表达的正性自动调节。我们的结论是，切不仅是必要的，但足够的感觉器官前体细胞及其后代的ES器官身份的规格。Cut功能对感觉器官细胞的特异性涉及前神经位点无子体和无毛鳞片复合体。
The loss of cut activity results in a change in neural identity in the peripheral nervous system so that the neurons and support cells of external sensory (es) organs are transformed into those of internal chordotonal (ch) organs. cut encodes a large nuclear homeo domain protein (Cut) that is expressed in the differentiated cells of es organs and their precursors but not in the cells of ch organs. We now analyze the effects of ectopic Cut expression in transformant lines of flies containing the Cut-coding sequences under inducible regulatory control. We demonstrate that ubiquitous Cut expression in embryos results specifically in the morphologic and antigenic transformation of ch organs into es organs. This effect appears to involve positive autoregulation of Cut expression. We conclude that Cut is not only necessary but sufficient for the specification of es organ identity in sensory organ precursor cells and their progeny. The specificity of Cut function to sensory organ cells involves the proneural loci daughterless and the achaete-scute complex.