Intraoperative Near-Infrared Fluorescence Imaging of Thymus in Preclinical Models.
Intraoperative Near-Infrared Fluorescence Imaging of Thymus in Preclinical Models.
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临床前模型中胸腺的术中近红外荧光成像。
DOI：
10.1016/j.athoracsur.2016.09.050
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
Frangioni,JohnV
中科院分区：
文献类型：
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作者：
Wada,Hideyuki;Hyun,Hoon;Kang,Homan;Gravier,Julien;Henary,Maged;Bordo,MarkW;Choi,HakSoo;Frangioni,JohnV
背景目前还没有胸腺特异性造影剂用于生物医学成像。因此，在某些手术中发现异位胸腺组织是非常困难的。本研究的目的是确定近红外（NIR）荧光成像是否可以提供高灵敏度，实时识别胸腺组织在operation.MethodsAfter初始在体内筛选的315-化合物NIR荧光团库胸腺摄取，将亚甲蓝和五种不同的700 nm发射候选分子注射到CD-1小鼠中，用于定量信号-背景比率作为动力学和剂量的函数。结果在35 kg约克郡猪中得到证实。双通道近红外成像也进行了使用各种800 nm发射近红外荧光团有针对性的各种组织在纵隔和neck.ResultsThe化合物恶嗪170表现出最高的信号-背景比（≥3）胸腺组织相对于纵隔脂肪，心脏，肺，肌肉，甲状腺和甲状旁腺，峰值信号背景比出现在1次静脉注射约7 mg的人体等效剂量后4小时。同时双通道近红外成像允许明确识别胸腺从周围组织，如内分泌腺和lymph nodes.ConclusionsIn小鼠和猪，近红外荧光成像使用恶嗪170允许高灵敏度，实时识别胸腺组织的外科手术需要切除或避免。目前尚不清楚恶嗪170用于人体胸腺组织成像的性能。
BackgroundThere are currently no thymus-specific contrast agents for biomedical imaging. Thus, finding ectopic thymic tissue during certain operations is extremely difficult. The purpose of the present study was to determine if near-infrared (NIR) fluorescence imaging could provide high sensitivity, real-time identification of thymic tissue during the operation.MethodsAfter initial in vivo screening of a 315-compound NIR fluorophore library for thymic uptake, methylene blue and five different 700-nm emitting candidate molecules were injected into CD-1 mice for quantitation of the signal-to-background ratio as a function of kinetics and dosing. Results were confirmed in 35-kg Yorkshire pigs. Dual-channel NIR imaging was also performed using a variety of 800-nm emitting NIR fluorophores targeted to various tissues in the mediastinum and neck.ResultsThe compound Oxazine 170 demonstrated the highest signal-to-background ratio (≥3) for thymic tissue relative to mediastinal fat, heart, lung, muscle, thyroid gland, and parathyroid gland, with peak signal-to-background ratio occurring 4 h after 1 intravenous injection of a human equivalent dose of approximately 7 mg. Simultaneous dual-channel NIR imaging permitted unambiguous identification of the thymus from surrounding tissues, such as endocrine glands and lymph nodes.ConclusionsIn mouse and pig, NIR fluorescence imaging using Oxazine 170 permits high sensitivity, real-time identification of thymic tissue for surgical procedures requiring its resection or avoidance. The performance of Oxazine 170 for imaging human thymic tissue is currently not known.