Impaired dendritic cell maturation in patients with chronic, but not resolved, hepatitis C virus infection
Impaired dendritic cell maturation in patients with chronic, but not resolved, hepatitis C virus infection
复制标题
DOI：
10.1182/blood.v97.10.3171
复制
发表时间：
2001-05-15
期刊：
影响因子：
20.3
通讯作者：
Levy, S
中科院分区：
文献类型：
--
作者：
Auffermann-Gratzinger, S;Keeffe, EB;Levy, S
树突状细胞（Dendritic cells，DC）是启动机体对外源抗原免疫应答的重要细胞。它们的抗原摄取和呈递能力使它们能够引发和激活T细胞。未成熟的DC捕获抗原;然而，它们必须在充当有效的抗原呈递细胞之前被激活以成熟。在病毒感染期间和肿瘤形成期间，DC的抗原提呈能力可能会减弱。丙型肝炎病毒（HCV）慢性感染已被证明会影响DCs的同种异体刺激功能。在这项研究中，它表明，单核细胞来源的DC从慢性HCV感染患者不响应成熟刺激。相反，它们保持其不成熟的表型，这反映在细胞表面标志物的模式和它们持续摄取抗原的能力上。此外，与来自健康供体的成熟DC相比，它们的同种异体刺激能力受损。为了研究病毒清除和这种DC成熟缺陷之间可能的相关性，研究了经过一个疗程的抗病毒治疗后解决HCV感染的患者。结果表明，清除HCV的患者的DC表现得像来自健康供体的DC：响应成熟刺激，它们减少抗原摄取，上调适当的表面标志物的表达，并且是同种异体T细胞的有效刺激物。（血。2001;97：3171-3176）（C）2001由美国血液学学会。
Dendritic cells (DCs) are important for the Initiation of immune responses to foreign antigens. Their antigen uptake and presentation capacities enable them to prime and activate T cells. Immature DCs capture antigens; however, they must be activated to mature before serving as efficient antigen-presenting cells. The antigen-presenting capacity of DCs can be diminished during viral infection and as a consequence of tumor formation. Chronic infection with hepatitis C virus (HCV) has been shown to affect the allostimulatory function of DCs. In this study, it is demonstrated that monocyte-derived DCs from patients with chronic HCV infection do not respond to maturation stimuli. Instead, they maintain their immature phenotype, reflected by the pattern of cell surface markers and by their continued capacity to uptake antigen. Moreover, their allostimulatory abilities are impaired compared with those of mature DCs derived from healthy donors. To investigate a possible correlation between viral clearance and this DC maturation defect, patients with resolved HCV infection after a course of antiviral therapy were studied. Results demonstrate that DCs from patients who cleared HCV behaved like DCs from healthy donors: in response to maturation stimuli, they decrease antigen uptake, up-regulate expression of appropriate surface markers, and are potent stimulators of allogeneic T cells. (Blood. 2001;97:3171-3176) (C) 2001 by The American Society of Hematology.