Gonadotropin-releasing hormone pulse frequency regulates expression of pituitary follistatin messenger ribonucleic acid: a mechanism for differential gonadotrope function.
Gonadotropin-releasing hormone pulse frequency regulates expression of pituitary follistatin messenger ribonucleic acid: a mechanism for differential gonadotrope function.
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DOI：
10.1210/endo.135.3.8070381
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发表时间：
1994-09
期刊：
影响因子：
4.8
通讯作者：
S. Kirk;A. Dalkin;M. Yasin;D. Haisenleder;J. Marshall
中科院分区：
文献类型：
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作者：
S. Kirk;A. Dalkin;M. Yasin;D. Haisenleder;J. Marshall
Follistatin （FS）是一种单体糖蛋白，可以选择性地抑制FSH的分泌和FSH β信使RNA （mRNA）的表达，可能是通过其结合激活素的能力。促性腺激素中存在FS mRNA和蛋白，提示其局部调控FSH β。垂体FS mRNA在性腺切除术后和发情周期促性腺激素高峰中期增加，与GnRH分泌增加的次数相同。因此，本研究的目的是评估GnRH分泌对垂体FS的调节作用。为了证实GnRH对FS的调节作用并研究性腺激素的作用，我们将成年雄性大鼠去腺（2-36 h），其中一些动物接受睾酮(T)替代，LRF-147（一种GnRH拮抗剂，AC-DTrp1-pCl-DPhe2-DTrp3-Ser4-Tyr5-DArg6-L eu7-Arg8-Pro9-DAla10），或两者同时接受36 h（从去势开始）。去势后垂体FS mRNA迅速升高，与未阉割动物相比，12 h升高3倍，36 h升高4倍（P = 120 min），选择性刺激FSH β mRNA升高，但不增加FS mRNA。这些结果表明垂体FS mRNA受GnRH的调控。此外，GnRH对垂体FS的频率调节提供了一种机制，即单个下丘脑GnRH可以不同地调节促性腺激素、LH和FSH。
Follistatin (FS) is a monomeric glycoprotein that selectively inhibits both secretion of FSH and expression of FSH beta messenger RNA (mRNA), presumably via its ability to bind activin. FS mRNA and protein are present in the gonadotrope, suggesting a local action in regulating FSH beta. Pituitary FS mRNA increases after gonadectomy and at the midcycle gonadotropin surge of the estrous cycle, times of increased GnRH secretion. Thus, the purpose of the present studies was to assess the role of GnRH secretion on the regulation of pituitary FS. To confirm GnRH regulation of FS and to study the role of gonadal steroids, adult male rats were gonadectomized (2-36 h), with some animals receiving either testosterone (T) replacement, LRF-147 (a GnRH antagonist, AC-DTrp1-pCl-DPhe2-DTrp3-Ser4-Tyr5-DArg6-L eu7-Arg8-Pro9-DAla10), or both for 36 h (from the time of castration). Pituitary FS mRNA increased rapidly after castration, with levels rising 3-fold by 12 h and 4-fold by 36 h when compared to intact animals (P or = 120 min), which selectively stimulated a rise in FSH beta mRNA, did not increase FS mRNA. These results indicate that pituitary FS mRNA is regulated by GnRH. In addition, GnRH frequency modulation of pituitary FS provides a mechanism whereby a single hypothalamic GnRH can differentially regulate the gonadotropins, LH and FSH.