Effect of phospholipid oxidation products on transbilayer movement of phospholipids in single lamellar vesicles.
Effect of phospholipid oxidation products on transbilayer movement of phospholipids in single lamellar vesicles.
复制标题
磷脂氧化产物对单层囊泡中磷脂跨双层运动的影响。
DOI：
10.1021/bi00534a017
复制
发表时间：
1982
期刊：
影响因子：
2.9
通讯作者：
Thompson,TE
中科院分区：
文献类型：
--
作者：
Shaw,JM;Thompson,TE
摘要：单层磷脂酰胆碱[Me/AyPHjcholine]囊泡与过量的未标记磷脂酰胆碱囊泡或含有8%葡萄糖醛酸二甘油酯的磷脂酰胆碱-胆固醇囊泡孵育。在存在或不存在纯化的磷脂酰胆碱交换蛋白的情况下，进行两个囊泡种群的孵育。DEAE-Sephacel柱层析可将带负电荷的糖脂供体囊泡完全去除。在与交换蛋白孵育和随后的分级后，-N(CD3)3磷脂酰胆碱受体囊泡显示出61-73%的未标记磷脂酰胆碱在外单分子层中的浓缩。在空气气氛中孵育时，至少5天内没有观察到外单层-N(CH3)3磷脂酰胆碱的明显跨双层运动。在两天之间
J. Michael Shaw1 and T. E. Thompson** abstract: Single lamellar phosphatidyl [me/AyPHjcholine vesicles were incubated with an excess of unlabeled phos-phatidylcholine vesicles or phosphatidylcholine-cholesterol vesicles containing 8 mol% glucuronosyldiglyceride. Incubation of the two vesicle populations was performed in the presence or absence of a purified phosphatidylcholineexchange protein. The negatively charged glycolipid donor vesicles could be completely removed by column chromatography on DEAE-Sephacel. Following incubation with exchange protein and subsequent fractionation, the-N (CD3) 3 phosphatidylcholine acceptor vesicles exhibited a 61-73% enrichment of the unlabeled phosphatidylcholine in the outer monolayer. Upon incubation in an air atmosphere, no appreciable trans-bilayer movement of the outer monolayer-N (CH3) 3 phos-phatidylcholine was observed for at least 5 days. Between days