SPATIAL AND TEMPORAL PATTERN OF HSP26 EXPRESSION DURING NORMAL DEVELOPMENT
SPATIAL AND TEMPORAL PATTERN OF HSP26 EXPRESSION DURING NORMAL DEVELOPMENT
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DOI：
10.1002/j.1460-2075.1986.tb04277.x
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发表时间：
1986-04-01
期刊：
影响因子：
11.4
通讯作者：
LIS, JT
中科院分区：
文献类型：
--
作者：
GLASER, RL;WOLFNER, MF;LIS, JT
利用β-lacZ的组织化学测定，在几个果蝇品系中分析了通过种系转化插入果蝇中的hsp 26-lacZ融合基因的发育表达的组织特异性模式。半乳糖苷酶活性。我们比较了这种模式的内源性热休克蛋白26 RNA的组织特异性分布，使用探针的RNA原位杂交在组织切片。这两种检测方法都表明，HSP 26在发育过程中的许多组织中表达，包括精母细胞，营养细胞，上皮细胞，成虫盘，腺胃和神经细胞。整个动物β的容易性和分辨率半乳糖苷酶测定使得其对于阐明参与这种复杂调节的序列特别有吸引力。原始的hsp 26-lacZ融合基因在转录起始点上游含有2kb的序列。一个只含有278 bp上游的结构仍然在精母细胞中表达，但不再在护士细胞。在少数情况下，融合基因在没有证据表明内源性hsp 26基因表达的组织中表达。这些新的模式似乎是染色体位置的结果，因为它们仅在一个或一个子集的含有相同插入片段的染色体系中观察到。
The tissue-specific patterns of developmental expression of hsp26-lacZ fusion genes inserted into Drosophila melanogaster by germline transformation were analyzed in several transformant lines utilizing a histochemical assay for .beta.-galactosidase activity on whole animals. We compared this pattern to the tissue-specific distribution of endogenous hsp26 RNA determined using hybridization of probes to RNA in situ in tissue sections. Both assays reveal that hsp26 is expressed in numerous tissues during development including spermatocytes, nurse cells, epithelium, imaginal discs, proventriculus and neurocytes. The ease and resolution of the whole-animal .beta.-galactosidase assay makes it particularly attractive for the elucidation of sequences involved in such complex regulation. The original hsp26-lacZ fusion gene contained 2 kb of sequence upstream of the transcription start. A construct containing only 278 bp upstream was still expressed in spermatocytes but no longer in nurse cells. In a few instances, the fusion genes were expressed in tissues for which there was no evidence for expression of the endogenous hsp26 gene. These novel patterns appear to be a result of chromosomal position since they were observed in only one or a subset of transformant lines containing identical inserts.