BDNF stimulates migration of cerebellar granule cells.
BDNF stimulates migration of cerebellar granule cells.
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发表时间：
2002-03
期刊：
影响因子：
4.6
通讯作者：
P. Borghesani;J. Peyrin;R. Klein;J. Rubin;Alexandre R. Carter;P. Schwartz;A. Luster;G. Corfas
中科院分区：
文献类型：
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作者：
P. Borghesani;J. Peyrin;R. Klein;J. Rubin;Alexandre R. Carter;P. Schwartz;A. Luster;G. Corfas
在神经系统的发育过程中，神经祖细胞在增殖区出现，然后退出细胞周期并从这些区域迁移。在这里，我们表明，迁移小脑颗粒细胞的增殖区，外部颗粒细胞层（EGL），在BDNF（-/-）小鼠受损。迁移受损的原因是BDNF直接和急性刺激颗粒细胞迁移。纯化的BDNF（-/-）颗粒细胞显示缺陷的迁移开始沿着胶质纤维和Boyden室测定。这种表型可以被外源性BDNF拯救。使用延时视频显微镜，我们发现BDNF是急性运动原性的，因为它刺激单个颗粒细胞的迁移后立即加入。迁移的刺激反映了BDNF的趋化动力学和趋化作用。总的来说，这些数据表明，BDNF是直接运动的颗粒细胞，并提供了一个方向性的线索，促进迁移从EGL的内部颗粒细胞层（IGL）。
During development of the nervous system, neural progenitors arise in proliferative zones, then exit the cell cycle and migrate away from these zones. Here we show that migration of cerebellar granule cells out of their proliferative zone, the external granule cell layer (EGL), is impaired in Bdnf(-/-) mice. The reason for impaired migration is that BDNF directly and acutely stimulates granule cell migration. Purified Bdnf(-/-) granule cells show defects in initiation of migration along glial fibers and in Boyden chamber assays. This phenotype can be rescued by exogenous BDNF. Using time-lapse video microscopy we find that BDNF is acutely motogenic as it stimulates migration of individual granule cells immediately after addition. The stimulation of migration reflects both a chemokinetic and chemotactic effect of BDNF. Collectively, these data demonstrate that BDNF is directly motogenic for granule cells and provides a directional cue promoting migration from the EGL to the internal granule cell layer (IGL).