Regional localization of the XRCC4 human radiation repair gene.
Regional localization of the XRCC4 human radiation repair gene.
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XRCC4 人类辐射修复基因的区域定位。
DOI：
10.1006/geno.1995.1029
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Stamato,TD
中科院分区：
文献类型：
--
作者：
Otevrel,T;Stamato,TD
为了定位XRCC 4人DNA修复基因亚染色体，对含有人5号染色体片段和pSV 2neo质粒的γ射线抗性人：XR-1仓鼠杂交细胞进行致死性照射，并与γ射线敏感的XR-1突变细胞融合。经G418抗性筛选，76个杂种中有2个保留了野生型γ射线抗性。使用来自两个耐药杂交体的DNA作为探针对正常人淋巴细胞进行FISH分析，在5 q13-q14产生了一个共同的杂交区域，这表明XRCC 4基因位于5号染色体的该区域。该基因物理定位在D5 S427和D5 S401微卫星标记之间，并且使用在人类染色体5中含有已知缺失的仓鼠：人类杂交种确认细胞学分配。
To map the XRCC4 human DNA repair gene subchromosomally, a gamma-ray-resistant human:XR-1 hamster hybrid cell containing fragments of human chromosome 5 and the pSV2neo plasmid was lethally irradiated and fused with the gamma-ray-sensitive XR-1 mutant cell. After selection for G418 resistance, 2 of a total of 76 hybrids retained wildtype gamma-ray resistance. FISH analysis of normal human lymphocytes using DNA from the two resistant hybrids as probes produced a common region of hybridization at 5q13-q14, suggesting that the XRCC4 gene is in this region of chromosome 5. The gene was physically localized between D5S427 and D5S401 microsatellite markers and the cytological assignment confirmed using hamster:human hybrids containing known deletions in human chromosome 5.