High-throughput protein crystallization
High-throughput protein crystallization
复制标题
DOI：
10.1016/s0959-440x(00)00131-7
复制
发表时间：
2000-10-01
影响因子：
6.8
通讯作者：
Stevens, RC
中科院分区：
文献类型：
--
作者：
Stevens, RC
组合化学工业在处理和混合小体积以及开发强大的液体处理系统方面取得了重大进展。此外，在高通量药物筛选和组合化学领域的物料处理领域也取得了进展。最后，同步加速器源的光束线光学的改进使快速冷冻微米尺寸（10-50微米）晶体的使用成为可能。这些和其他最新进展的结合将使高通量蛋白质晶体学成为可能。蛋白质晶体学的高通量方法的进一步发展将需要应用上述发展，并以更系统的方式积累成功/失败数据。基于第一代高通量系统收集的数据，晶体学技术将出现重大变化。
The combinatorial chemistry industry has made major advances in the handling and mixing of small volumes, and in the development of robust liquid-handling systems. In addition, developments have been made in the area of material handling for the high-throughput drug screening and combinatorial chemistry fields. Lastly, improvements in beamline optics at synchrotron sources have enabled the use of flash-frozen micron-sized (10-50 mum) crystals. The combination of these and other recent advances will make high-throughput protein crystallography possible. Further advances in high-throughput methods of protein crystallography will require application of the above developments and the accumulation of success/failure data in a more systematic manner. Major changes in crystallography technology will emerge based on the data collected by first-generation high-throughput systems.