Jelleines:: a family of antimicrobial peptides from the Royal Jelly of honeybees (Apis mellifera)
Jelleines:: a family of antimicrobial peptides from the Royal Jelly of honeybees (Apis mellifera)
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DOI：
10.1016/j.peptides.2004.03.016
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发表时间：
2004-06-01
期刊：
影响因子：
3
通讯作者：
Palma, MS
中科院分区：
文献类型：
--
作者：
Fontana, R;Mendes, MA;Palma, MS
采用反相高效液相色谱法从蜜蜂皇家果冻中分离纯化了4种抗菌肽，并采用Q-Tof-MS/MS进行序列测定：PFKLSLHL-NH 2（Jelleine-I）、TPFKLSLHL-NH 2（Jelleine-II）、EPFKLSLHL-NH 2（Jelleine-III）和TPFKLSLH-NH 2（Jelleine-IV）。在固相上合成肽，纯化并进行不同的生物测定：抗微生物活性、肥大细胞脱粒活性和溶血。Jelleine-I-III仅对酵母菌、革兰氏阳性菌和革兰氏阴性菌具有抗微生物活性;同时，Jelleine-IV在所进行的所有试验中均无活性。这些肽与来自蜜蜂的其他抗微生物肽没有任何相似性;它们由工蜂组成性产生并分泌到皇家果冻中。(C)2004年爱思唯尔公司All rights reserved.
Four antimicrobial peptides were purified from Royal Jelly of honeybees, by using reverse phase-HPLC and sequenced by using Q-Tof-MS/MS: PFKLSLHL-NH2 (Jelleine-I), TPFKLSLHL-NH2 (Jelleine-II), EPFKLSLHL-NH2 (Jelleine-III), and TPFKLSLH-NH2 (Jelleine-IV). The peptides were synthesized on-solid phase, purified and submitted to different biological assays: antimicrobial activity, mast cell degranulating activity and hemolysis. The Jelleines-I-III presented exclusively antimicrobial activities against yeast, Gram+ and Gram- bacteria; meanwhile, Jelleine-IV was not active in none of the assays performed. These peptides do not present any similarity with the other antimicrobial peptides from the honeybees; they are produced constitutively by the workers and secreted into Royal Jelly. (C) 2004 Elsevier Inc. All rights reserved.