Exercise pretraining attenuates endotoxin-induced hemodynamic alteration in type I diabetic rats
Exercise pretraining attenuates endotoxin-induced hemodynamic alteration in type I diabetic rats
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DOI：
10.1139/h08-081
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发表时间：
2008-10-01
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影响因子：
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通讯作者：
Cheng, Juei-Tang
中科院分区：
文献类型：
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作者：
Hung, Ching-Hsia;Chen, Yu-Wen;Cheng, Juei-Tang
热休克蛋白72 （HSP72）的高表达降低了脓毒性休克的死亡率和器官损伤，并防止了脂多糖（LPS）引起的心脏线粒体功能障碍。我们的假设是，运动预处理可能增加心脏和脑孤束核（NTS）中HSP72的表达，从而减轻内毒素治疗的1型糖尿病大鼠的心血管功能障碍。Wistar大鼠被随机分为以下三组：久坐的正常大鼠、久坐的1型糖尿病大鼠和运动训练的1型糖尿病大鼠。经过训练的大鼠在跑步机上跑步，每周5天（-1），每天30-60分钟（-1），强度为每小时1.0英里（-1英里= 1.6公里），持续3周。在最后一次训练24小时后，我们比较了注射LPS的大鼠的平均动脉压、心率、心输出量、中风量和血清肿瘤坏死因子a水平的时间分布。检测各组大鼠心脏和NTS组织中HSP72的表达。我们发现，运动训练后，糖尿病大鼠心脏和NTS中的HSP72表达显著增加。给药后，运动训练的糖尿病大鼠存活时间明显延长。此外，与未接受运动训练的大鼠相比，血清肿瘤坏死因子α水平下降。运动训练还能减轻内毒素血症期间糖尿病大鼠的心血管功能障碍。这些数据表明，运动可能增加心脏和NTS中HSP72的表达，以防止内毒素血症期间糖尿病大鼠的高死亡率和减轻心血管功能障碍。
Higher expression of heat shock protein 72 (HSP72) reduces the mortality rate and organ damage in septic shock and prevents cardiac mitochondrial dysfunction due to lipopolysaccharide (LPS). Our hypothesis is that exercise preconditioning may increase the expression of HSP72 in heart and the nucleus tractus solitarii (NTS) of the brain to alleviate the cardiovascular dysfunction in type I diabetic rats receiving endotoxin. Wistar rats were randomly assigned to the following groups: sedentary normal, sedentary type I diabetic rats, and type I diabetic rats with exercise training. The trained rats ran on a treadmill 5 d.week(-1), 30-60 min.d(-1), at an intensity of 1.0 mile.h(-1) (1 mile = 1.6 km) over a 3 week period. Twenty-four hours after the last training session, we compared the temporal profiles of mean arterial pressure, heart rate, cardiac output, stroke volume, and serum tumor necrosis factor a level in rats receiving an injection of LPS. In addition, HSP72 expression in heart and NTS from each group was determined. We found that HSP72 expression in the heart and NTS was significantly increased in diabetic rats with exercise training. After administration of LPS, the survival time was significantly longer in diabetic rats with exercise training. Additionaly, serum tumor necrosis factor alpha levels decreased as compared with those rats not receiving exercise training. Exercise training also diminished cardiovascular dysfunction in diabetic rats during endotoxemia. These data suggest that exercise may increase the expression of HSP72 in the heart and NTS to protect against the high mortality rate and attenuate cardiovascular dysfunction in diabetic rats during endotoxemia.