Preparation of colloidal gold for staining proteins electrotransferred onto nitrocellulose membranes.
Preparation of colloidal gold for staining proteins electrotransferred onto nitrocellulose membranes.
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DOI：
10.1016/0003-2697(88)90417-4
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发表时间：
1988-07
影响因子：
2.9
通讯作者：
K. Yamaguchi;H. Asakawa
中科院分区：
文献类型：
--
作者：
K. Yamaguchi;H. Asakawa
本文介绍了一种制备胶体金的简单方法。以0.005%和0.01%的HAuCl_4为原料，加入福尔马林还原剂和氢氧化钾制备胶体金。用这种胶体金溶液将小细胞癌组织提取物印迹到硝酸纤维素膜上进行染色，导致出现大量清晰的酒红色条带。金染色的敏感性比考马斯亮蓝染色高60倍，与聚丙烯酰胺凝胶蛋白质银染色的敏感性相当。与酶免疫印迹法比较，该方法的灵敏度也令人满意。该方法制备的胶体金可用于日常工作。
This paper describes a simple method of preparing colloidal gold for staining protein blots. Colloidal gold was prepared from 0.005 or 0.01% HAuCl4by the addition of formalin as a reductant and potassium hydroxide. Staining of small cell carcinoma tissue extract blotted onto nitrocellulose membranes with this colloidal gold solution resulted in the appearance of a large number of clear wine-red bands. The sensitivity of gold staining was 60 times higher than that of Coomassie brilliant blue staining and almost comparable to that of silver staining of proteins in polyacrylamide gel. The sensitivity of this method was also satisfactory in comparison with that of enzyme immunoblotting. The colloidal gold prepared by this method is usable for routine work.