Structure of the manganese superoxide dismutase from Deinococcus radiodurans in two crystal forms
Structure of the manganese superoxide dismutase from Deinococcus radiodurans in two crystal forms
复制标题
DOI：
10.1107/s1744309106008402
复制
发表时间：
2006-04-01
影响因子：
0.9
通讯作者：
McSweeney, S
中科院分区：
文献类型：
--
作者：
Dennis, RJ;Micossi, E;McSweeney, S
测定了耐辐射球菌锰超氧化物歧化酶（Mn-SOD; DR 1279）两种不同晶型的结构。两种晶体都是单斜晶系，空间群为P2（1）。形式I具有晶胞参数a = 44.28，B = 83.21，c = 59.52埃，β = 110.18度，并且在不对称单元中含有同二聚体，使用d（min）= 2.2埃的数据进行结构精修（R = 16.8%，无R = 23.6%）。形式II具有晶胞参数a = 43.57，B = 87.10，c = 116.42埃，β = 92.1度和含有两个Mn-SOD同源二聚体的不对称单元;结构精修达到2.0埃的分辨率（R = 17.2%，无R = 22.3%）。所得的结构进行了比较，从大肠杆菌，它们被证明是基本上是同构的Mn-SOD。
The structure of the manganese superoxide dismutase (Mn-SOD; DR1279) from Deinococcus radiodurans has been determined in two different crystal forms. Both crystal forms are monoclinic with space group P2(1). Form I has unit-cell parameters a = 44.28, b = 83.21, c = 59.52 angstrom, beta = 110.18 degrees and contains a homodimer in the asymmetric unit, with structure refinement (R = 16.8%, R-free = 23.6%) carried out using data to d(min) = 2.2 angstrom. Form II has unit-cell parameters a = 43.57, b = 87.10, c = 116.42 angstrom, beta = 92.1 degrees and an asymmetric unit containing two Mn-SOD homodimers; structure refinement was effected to a resolution of 2.0 angstrom (R = 17.2%, R-free = 22.3%). The resulting structures are compared with that of Mn-SOD from Escherichia coli, with which they are shown to be essentially isostructural.