KiT: a MATLAB package for kinetochore tracking.
KiT: a MATLAB package for kinetochore tracking.
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DOI：
10.1093/bioinformatics/btw087
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发表时间：
2016-06-15
期刊：
影响因子：
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通讯作者：
Burroughs NJ
中科院分区：
文献类型：
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作者：
Armond JW;Vladimirou E;McAinsh AD;Burroughs NJ
摘要：在有丝分裂过程中，染色体通过称为动粒的大蛋白质复合物附着在有丝分裂纺锤体上。有丝分裂过程中动粒的运动是复杂的，对它们运动的自动定量跟踪已经揭示了它们行为的许多令人惊讶的方面。在这里，我们提出了“KiT”（动粒跟踪）-一个易于使用的，开源的软件包，用于跟踪动粒从活细胞荧光电影。KiT支持2D，3D和多色电影，荧光定量，集成反卷积，并行执行和多种粒子定位算法。可用性和实施：KiT是在MATLAB中实现的免费开源软件，可在所有MATLAB支持的平台上运行。KiT可以从http://www.mechanochemistry.org/mcainsh/software.php下载。源代码库可在https://bitbucket.org/jarmond/kit上获得，并正在继续开发中。补充信息：补充数据可从在线生物信息学获得。联系人：jonathan. warwick.ac.uk
Summary: During mitosis, chromosomes are attached to the mitotic spindle via large protein complexes called kinetochores. The motion of kinetochores throughout mitosis is intricate and automated quantitative tracking of their motion has already revealed many surprising facets of their behaviour. Here, we present ‘KiT’ (Kinetochore Tracking)—an easy-to-use, open-source software package for tracking kinetochores from live-cell fluorescent movies. KiT supports 2D, 3D and multi-colour movies, quantification of fluorescence, integrated deconvolution, parallel execution and multiple algorithms for particle localization. Availability and implementation: KiT is free, open-source software implemented in MATLAB and runs on all MATLAB supported platforms. KiT can be downloaded as a package from http://www.mechanochemistry.org/mcainsh/software.php. The source repository is available at https://bitbucket.org/jarmond/kit and under continuing development. Supplementary information: Supplementary data are available at Bioinformatics online. Contact: jonathan.armond@warwick.ac.uk