Fluoro-Jade B histofluorescence staining detects dentate granule cell death after repeated five-minute transient global cerebral ischemia
Fluoro-Jade B histofluorescence staining detects dentate granule cell death after repeated five-minute transient global cerebral ischemia
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DOI：
10.1007/s11011-019-00404-5
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发表时间：
2019-06-01
影响因子：
3.6
通讯作者：
Park, Joon Ha
中科院分区：
文献类型：
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作者：
Ahn, Ji Hyeon;Ohk, Taek Geun;Park, Joon Ha
短暂性脑缺血（tGCI）引起的神经元损伤随其持续时间和程度的不同而变化。关于tGCI鼠模型海马锥体细胞（CA1-3）损伤或死亡的研究较多。然而，关于tGCI后海马齿状回颗粒细胞死亡的研究尚未得到解决。本研究采用甲酚紫染色、NeuN免疫组化和Fluoro-Jade B （F-J B）组织荧光染色，观察单次tGCI和重复tGCI（两次tGCI，每次5分钟，间隔1小时）不同持续时间（5,10和15min）后5d沙鼠DG颗粒细胞的损伤/死亡情况。在所有单一tgci手术组中，仅在多态层观察到神经元死亡。而在重复tgci手术组，F-J - B组织荧光染色观察到颗粒细胞层和多态层大量神经元死亡。此外，与tgci手术15分钟组相比，重复tgci手术组DG的小胶质瘤明显增加。综上所述，这些发现表明，重复的短暂tGCI导致DG颗粒细胞死亡，而单次tGCI持续时间较长则不会发生这种情况。
Transient global cerebral ischemia (tGCI)-induced neuronal damage is variable according to its duration and degree. There are many studies on the damage or death of pyramidal cells of the hippocampus proper (CA1-3) in rodent models of tGCI. However, studies on the death of granule cells in the hippocampal dentate gyrus (DG) following tGCI have not yet been addressed. In this study, we examined the damage/death of granule cells in the gerbil DG at 5days after various durations (5, 10, and 15min) of single tGCI and repeated tGCI (two 5-min tGCI with 1-h interval) using cresyl violet staining, NeuN immunohistochemistry and Fluoro-Jade B (F-J B) histofluorescence staining. Neuronal death was observed only in the polymorphic layer in all single tGCI-operated groups. However, in the repeated tGCI-operated group, massive neuronal death was observed in the granule cell layer as well as in the polymorphic layer by using F-J B histofluorescence staining. In addition, microgliosis in the DG was significantly increased in the repeated tGCI-operated group compared to the 15-min tGCI-operated group. Taken together, these findings indicate that repeated brief tGCI causes granule cell death in the DG which could not occur by a longer duration of single tGCI.