The release of substance P from cultured dorsal root ganglion neurons requires the non-neuronal cells around these neurons
The release of substance P from cultured dorsal root ganglion neurons requires the non-neuronal cells around these neurons
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DOI：
10.1254/jphs.fp0071359
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发表时间：
2007-11-01
影响因子：
3.5
通讯作者：
Nakata, Yoshihiro
中科院分区：
文献类型：
--
作者：
Tang, He-Bin;Li, Yu-Sang;Nakata, Yoshihiro
已知P物质从背根神经节（DRG）神经元释放，但在P物质释放过程中可能涉及非神经元细胞尚不清楚。在培养期间，监测存活的DRG神经元的数量、培养液和DRG细胞中的总P物质含量以及DRG细胞的总蛋白。存活的神经元数量和总P物质含量均呈时间依赖性下降，而总蛋白质合成增加。免疫细胞化学进一步证实了P物质在中小型神经元中的定位。最后，通过放射免疫测定法研究了神经元和非神经元富集物中P物质的释放水平。相对于未纯化的DRG细胞，部分纯化的DRG神经元和非神经元细胞对辣椒素或KCl的P物质释放反应较弱。部分纯化的DRG神经元的总P物质含量与未纯化的DRG细胞几乎相同，但远高于部分纯化的DRG非神经元细胞。这些发现表明，P物质是从DRG神经元释放，这一过程应该需要神经元和非神经元细胞的共存。
Substance P is known to be released from dorsal root ganglion (DRG) neurons, but the possible involvement of non-neuronal cells in the process of substance P release is not known. During the culture period, the number of surviving DRG neurons, the total substance P content from the culture medium and DRG cells, and the total protein of DRG cells were monitored. Both the number of surviving neurons and the total substance P content decreased in a time-dependent manner, whereas the total protein synthesis was increased. The localization of substance P in small-to-medium-sized neurons was further confirmed by immunocytochemistry. Finally, the substance P release levels from the neuronal and non-neuronal enrichments were investigated by radioimmunoassay. Both partially purified DRG neurons and non-neuronal cells exhibited a weaker substance P release response to capsaicin or KCl, relative to unpurified DRG cells. The total substance P content from the partially purified DRG neurons was almost the same as that from the unpurified DRG cells, but much more than that from the partially purified DRG non-neuronal cells. These findings suggest that substance P is released from DRG neurons, and this process should require the coexistence of neurons and non-neuronal cells.