Lansoprazole induces collagenous colitis in the colon of Mongolian gerbils.
Lansoprazole induces collagenous colitis in the colon of Mongolian gerbils.
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DOI：
10.1016/s0016-5085(11)62698-x
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发表时间：
2011-05
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影响因子：
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通讯作者：
Takashi Mizushima;T. Mizoshita;M. Sasaki;S. Tanida;Hironobu Tsukamoto;T. Shimura;T. Kanematsu;H. Kataoka;T. Kamiya;T. Tsukamoto;M. Tatematsu;T. Joh
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文献类型：
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作者：
Takashi Mizushima;T. Mizoshita;M. Sasaki;S. Tanida;Hironobu Tsukamoto;T. Shimura;T. Kanematsu;H. Kataoka;T. Kamiya;T. Tsukamoto;M. Tatematsu;T. Joh
胶原性结肠炎（CC）是一种以慢性腹泻为特征的疾病，可能对肿瘤的发展产生影响，但在动物中尚无报道。因此，我们试图通过长期连续给予兰索拉唑（LPZ）在蒙古沙土鼠（MG）模型中建立CC发展，旨在阐明LPZ给药与CC发生之间的关系。将69只沙鼠分为6组：幽门螺杆菌（Hp）感染+高剂量LPZ组、Hp感染+低剂量LPZ组、Hp感染+高剂量LPZ组、低剂量LPZ组和对照组。在实验第27、54和108周处死沙鼠并切除整个结肠。我们检查了结肠病变的瑞士卷肠染色病理。共有3只沙鼠在近端结肠有CC样病变。所有具有CC样病变的MG均来自LPZ给药组（3/35; 8.6%）。在这些病变中检测到的增厚的上皮下胶原带与人类CC病变非常相似。免疫组化分析表明，更多的嗜铬粒蛋白A阳性细胞在结肠隐窝上层以下连续LPZ管理的趋势。总之，我们通过连续给予LPZ成功地建立了MG模型中CC样病变的发展，并确定了由LPZ给药诱导的异位内分泌细胞可能影响结肠中这些病变的发生。
Collagenous colitis (CC) is an illness characterized by chronic diarrhea with possible effects on neoplastic development, but there have been no reports in animals. We therefore tried to establish CC development in a Mongolian gerbil (MG) model by long-term continuous lansoprazole (LPZ) administration and aimed to clarify the relationship between LPZ administration and CC occurrence. We divided 69 gerbils into 6 groups: Helicobacter pylori (Hp)-infected+high-dose LPZ, Hp-infected+low-dose-LPZ, Hp-infected, high-dose-LPZ, low-dose-LPZ, and control. The gerbils were sacrificed and entire colons were excised at experimental weeks 27, 54, and 108. We examined colonic lesions by staining of Swiss-roll intestines pathologically. A total of 3 gerbils had CC-like lesions in the proximal colon. All MGs with CC-like lesions were from LPZ treated groups (3 of 35; 8.6%). The thickened subepithelial collagen band detected in these lesions strongly resembled that of human CC lesions. Immunohistochemical analysis indicated a tendency for more chromogranin A-positive cells in the upper layer of colonic crypt following continuous LPZ administration. In conclusion, we successfully established development of CC-like lesions in an MG model by continuous LPZ administration and determined that the ectopic endocrine cells that were induced by LPZ administration may influence the occurrence of these lesions in the colon.