Corrected emission spectra and quantum yields for a series of fluorescent compounds in the visible spectral region
Corrected emission spectra and quantum yields for a series of fluorescent compounds in the visible spectral region
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DOI：
10.1023/b:jofl.0000031828.96368.c1
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发表时间：
2004-07-01
影响因子：
2.7
通讯作者：
Tonnesen, HH
中科院分区：
文献类型：
--
作者：
Velapoldi, RA;Tonnesen, HH
越来越多地使用稳态和动态荧光测量来解决化学问题，这直接归因于其灵敏度和选择性以及测量系统的创新和发展。此外，在过去的二十年里，荧光在生化测量问题中的应用已经大大增加，特别是通过新的荧光配体的开发和它们在抗体技术中的应用。然而，在这些发展和应用中，准确的测量和不同实验室之间结果的可比性是持续进步的关键，并且依赖于用于稳态和动态测量的荧光标准的可用性。重要的是，实验室，特别是国家标准实验室，制定研究实验室可以使用的荧光标准，以便研究实验室能够将精力集中在测量、应用和化学突破上，而不是制定标准以确保其测量的准确性和可转移性。20世纪70年代末发布了第一个荧光标准物质--二水硫酸奎宁(SRM936)[1]，这是朝着这个方向迈出的一步[2]，随后研讨会上不断有报告，其中包括继续需要和研究更多的荧光标准[3]。Magde等人[4]最近通过罗丹明6G和荧光素在各种溶剂中的热晕技术提供了绝对量子产率。他们还使用这些数据和更早报告的寿命[5]来调用-
The increased use of steady-state and dynamic fluorescence measurements to address chemical problems is directly attributable to its sensitivity and selectivity as well as innovations and developments in measurement systems. Additionally, during the last two decades fluorescence applications to biochemical measurement problems have increased tremendously, especially through the development of new fluorescent ligands and their inclusion in antibody techniques.In these developments and applications, however, accurate measurements and comparability of results among various laboratories are critical for continued progress and are dependent on the availability of fluorescence standards for both steady-state and dynamic measurements. It is important that laboratories, especially National Standards Laboratories, develop fluorescence standards that research laboratories can use so that the research laboratories can focus their efforts on measurements, applications and chemical breakthroughs, rather than developing standards to ensure that their measurements are accurate and transferable. The issuance [1] of the first fluorescence Standard Reference Material, Quinine Sulfate Dihydrate (SRM 936) in the late 1970’s [2] was a step in this direction and has been followed by reports at workshops that included the continuing need for and research on additional fluorescence standards [3]. Magde et al.[4] have recently provided absolute quantum yields by a thermal blooming technique for rhodamine 6g and fluorescein in various solvents. They also used these data with earlier reported lifetimes [5] to cal-