An in vitro study of differentiation of hematopoietic cells to endothelial cells.
An in vitro study of differentiation of hematopoietic cells to endothelial cells.
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DOI：
10.1155/2011/846096
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Yan Q
中科院分区：
文献类型：
--
作者：
Wang QR;Wang BH;Zhu WB;Huang YH;Li Y;Yan Q
骨髓来源的血管内皮祖细胞(BM-EPC)可促进出生后新生血管的形成，因此在细胞疗法治疗缺血性疾病方面具有重要意义。然而，它们的起源和特点仍存在争议。本文对从骨髓单个核细胞中分离并在骨髓内皮细胞条件培养液(ECCM)诱导下分化的BM-EPC的来源/谱系进行了鉴定。从表型、谱系潜能和功能特性方面对BM-EPC进行了鉴定。BM-EPC来源的内皮细胞克隆与ECCM共同培养3个月。体外培养的EPC集落细胞表达内皮细胞标志物，并形成毛细血管样网络。EPC集落细胞表现出不同的增殖能力；一些集落表现出高增殖潜能(HPP)，最高可达20个群体倍增。更重要的是，这些HPP-EPC表达造血标记物CD45，具有内吞活性，并保留了部分髓系细胞的活性。此外，HPP-EPC还能分泌多种生长因子，包括血管内皮生长因子和粒细胞集落刺激因子。结果表明，这些内皮祖细胞主要来源于早期前体细胞的造血源，并保持了较高的增殖能力，这一特点在细胞治疗缺血性疾病中具有重要的应用前景。
Bone-marrow-derived endothelial progenitor cells (BM-EPCs) contribute to postnatal neovascularization and therefore are of great interest for cell therapies to treat ischemic diseases. However, their origin and characteristics are still in controversy. In this paper, we identified the origin/lineage of the BM-EPCs that were isolated from bone marrow mononuclear cells and differentiated with the induction of bone-marrow endothelial-cellconditioned medium (ECCM). BM-EPCs were characterized in terms of phenotype, lineage potential, and their functional properties. Endothelial cell colonies derived from BM-EPC were cultured with ECCM for 3 months. Cultured EPC colony cells expressed endothelial cell markers and formed the capillary-like network in vitro. EPC colony cells expressed differential proliferative capacity; some of the colonies exhibited a high proliferative potential (HPP) capacity up to 20 population doublings. More importantly, these HPP-EPCs expressed hematopoietic marker CD45, exhibited endocytic activities, and preserved some of the myeloid cell activity. In addition, the HPP-EPCs secrete various growth factors including VEGF and GM-CSF into the culture medium. The results demonstrate that these EPCs were primarily derived from hematopoietic origin of early precursor cells and maintained high proliferative potential capacity, a feature with a significant potential in the application of cell therapy in ischemic diseases.