Study on the cell cycle of Bacillus subtilis using temperature-sensitive mutants
Study on the cell cycle of Bacillus subtilis using temperature-sensitive mutants
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利用温度敏感突变体研究枯草芽孢杆菌的细胞周期
DOI：
10.1007/bf00268428
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
T. Komano
中科院分区：
文献类型：
--
作者：
Y. Miyakawa;T. Komano
从枯草芽孢杆菌168的诱变群体中随机分离的434个温度敏感突变体中鉴定出3个（ts 31、ts 341、ts 526）温度敏感突变体，这些突变体在隔膜形成方面有缺陷，并且在非允许温度下形成无隔膜的丝状体。形态学观察和鉴定结果表明，ts 31和ts 341是隔膜起始突变体，ts 526是DNA延长突变体。通过PBS 1介导的转导将上述突变和影响隔膜起始的其它突变（div 355和tms 12）定位在染色体上的如下三个独立区域中：pur A16-ts 526-div 355-cysA 14; metC 3-（ts 31，tms 12）-pyrD 1-recA 1; ebr-2-ts 341-uvrA 1-hisA 1-cysB 3。我们的结果表明，隔膜形成的启动过程至少需要四种基因产物。此外，ts 526的结果表明，隔膜起始和DNA复制之间的密切联系。
Among temperature-sensitive mutants which were defective in septum formation and formed nonseptate filaments at nonpermissive temperatures three (ts31, ts341, ts526) were identified among 434 temperature-sensitive mutants isolated at random from a mutagenized population ofBacillus subtilis168. The results of morphological observations and characterization of these mutants showed that ts31 and ts341 were septum-initiation mutants and that ts526 was a DNA elongation mutant. The above mutations, and other mutations affecting septum initiation (div355andtms12) were mapped by PBS1-mediated transduction on the chromosome in three separate regions as follows:pur A16-ts526-div355-cysA14; metC3-(ts31, tms12)-pyrD1-recA1; ebr-2-ts341-uvrA1-hisA1-cysB3. Our results suggest that the initiation process of septum formation requires at least four kinds of gene product. In addition, the sesult obtained with ts526 suggests an intimate connection between septum initiation and DNA replication.