OsRap2.6 transcription factor contributes to rice innate immunity through its interaction with Receptor for Activated Kinase-C 1 (RACK1).
OsRap2.6 transcription factor contributes to rice innate immunity through its interaction with Receptor for Activated Kinase-C 1 (RACK1).
复制标题
OSRAP2.6转录因子通过与激活的激酶-C 1（RACK1）的受体相互作用来促进水稻先天免疫。
DOI：
10.1186/1939-8433-5-35
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发表时间：
2012-12-11
期刊：
影响因子：
--
通讯作者：
Shimamoto K
中科院分区：
文献类型：
--
作者：
Wamaitha MJ;Yamamoto R;Wong HL;Kawasaki T;Kawano Y;Shimamoto K
水稻小分子GTP酶Osrac1是水稻天然免疫中的一个分子开关。激活的激酶C-1受体(RACK1)与Osrac1相互作用，抑制稻瘟病菌的生长。RACK1在水稻中有两个同源物，RACK1A和RACK1B。过表达RACK1A提高了对稻瘟病菌的抗性。然而，RACK1A下游信号在很大程度上是未知的。在这里，我们报告了与RACK1A相互作用的转录因子OsRap2.6的鉴定。我们发现OsRap2.6AP2结构域与拟南芥Rap2.6(AtRap2.6)有94%的相似性。用带有金星C-端和N-端片段的标记OsRap2.6和RACK1A对水稻原生质体进行双分子荧光互补(BIFC)分析表明，OsRap2.6和RACK1A相互作用，定位于细胞核和细胞质中。此外，OsRap2.6和OsMAPK3/6在胞核和胞浆中相互作用。甲壳素处理诱导了防御基因PAL1、PBZ1和OsRap2.6的表达。对感染稻瘟菌的OsRap2.6RNAi和过量表达(Ox)植株进行的抗病性分析表明，OsRap2.6RNAi植株对稻瘟病高度敏感，而OsRap2.6Ox植株对亲和性稻瘟菌的抗性增强。OsRap2.6通过与RACK1A在亲和作用中的相互作用促进水稻的先天免疫。本文的在线版本(DOI：10.1186/1939-8433-5-35)包含补充材料，可供授权用户使用。
The rice small GTPase OsRac1 is a molecular switch in rice innate immunity. The Receptor for Activated Kinase C-1 (RACK1) interacts with OsRac1 to suppress the growth of the rice blast fungus, Magnaporthe oryzae. RACK1 has two homologs in rice, RACK1A and RACK1B. Overexpressing RACK1A enhances resistance to the rice blast fungus. However, RACK1A downstream signals are largely unknown. Here, we report the identification of OsRap2.6, a transcription factor that interacts with RACK1A. We found a 94% similarity between the OsRap2.6 AP2 domain and Arabidopsis Rap2.6 (AtRap2.6). Bimolecular fluorescence complementation (BiFC) assays in rice protoplasts using tagged OsRap2.6 and RACK1A with the C-terminal and N-terminal fragments of Venus (Vc/Vn) indicated that OsRap2.6 and RACK1A interacted and localized in the nucleus and the cytoplasm. Moreover, OsRap2.6 and OsMAPK3/6 interacted in the nucleus and the cytoplasm. Expression of defense genes PAL1 and PBZ1 as well as OsRap2.6 was induced after chitin treatment. Disease resistance analysis using OsRap2.6 RNAi and overexpressing (Ox) plants infected with the rice blast fungus indicated that OsRap2.6 RNAi plants were highly susceptible, whereas OsRap2.6 Ox plants had an increased resistance to the compatible blast fungus. OsRap2.6 contributes to rice innate immunity through its interaction with RACK1A in compatible interactions. The online version of this article (doi:10.1186/1939-8433-5-35) contains supplementary material, which is available to authorized users.