Blood permeability of a novel ceramic scaffold for bone morphogenetic protein-2
Blood permeability of a novel ceramic scaffold for bone morphogenetic protein-2
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DOI：
10.1002/jbm.b.30686
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发表时间：
2007-05-01
影响因子：
3.4
通讯作者：
Arisue, M.
中科院分区：
文献类型：
--
作者：
Murata, M.;Akazawa, T.;Arisue, M.
以牛骨为原料制备了具有体液渗透性的功能梯度磷灰石（fg-HAp）。采用组织形态计量学方法评价fg-HAp的组织反应及其作为重组人骨形态发生蛋白-2（BMP-2）支架的有效性，免疫印迹法分析fg-HAp渗透液的A a组分。将fg-HAp块（27 mm（3））与BMP-2（5 μ g）联合或不联合植入4周龄Wistar大鼠皮下。组织学检查显示，fg-HAp的表面和本体降解广泛进行，在2周时，fg-HAp上出现巨细胞。在fg-HAp内发现体液渗透，并且液体组分对白蛋白呈免疫阳性。此外，在从体内植入的fg-HAp收集的蛋白质中，检测到白蛋白作为主要成分。随着BMP-2诱导的骨成熟，fg-HAp的生物吸收加速。BMP-2/fg-HAp植入物4周时的组织形态计量学分析显示，骨和骨髓的总体积为59.0%。这些结果表明，fg-HAp是一种创新的，生物可吸收的生物陶瓷，具有流体渗透特性，并可能成为一种生物整合的骨工程支架。(c)2006 Wiley Periodicals，Inc.
A functionally graded apatite (fg-HAp) with body fluid permeability was developed from bovine bone. The tissue reaction of fg-HAp and its efficacy as a scaffold for recombinant human bone morphogenetic protein-2 (BMP-2) were evaluated histomorphometrically, a A a component of permeable fluid into the fg-HAp was analyzed by immunoblotting assay. The fg-HAp block (27 mm(3)) combined with and without BMP-2 (5 mu g) was implanted subcutaneously in 4-week-old Wistar rats. Histological examination showed that the surface and bulk degradations of the fg-HAp proceeded extensively and giant cells appeared on the fg-HAp at 2 weeks. Body fluid permeation was found inside the fg-HAp, and the fluid component was immunopositive for albumin. In addition, albumin was detected as a main component among proteins collected from the in vivo implanted fg-HAp. The bioabsorption of the fg-HAp was accelerated as BMP-2-induced bone matured. Histomorphometrical analysis at 4 weeks in the BMP-2/fg-HAp implant showed 59.0% in the total volume of bone and marrow. These results indicate that fg-HAp is an innovative, bioabsorbable bioceramic with fluid permeability characteristic, and may become a biointegrated scaffold for bone engineering. (c) 2006 Wiley Periodicals, Inc.