Optimization of methanol biosynthesis byMethylosinus trichosporium OB3b: An approach to improve methanol accumulation
Optimization of methanol biosynthesis byMethylosinus trichosporium OB3b: An approach to improve methanol accumulation
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毛孢甲基窦菌 OB3b 甲醇生物合成的优化：提高甲醇积累的方法
DOI：
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发表时间：
1997
期刊：
影响因子：
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通讯作者：
I. Okura
中科院分区：
文献类型：
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作者：
Masayuki Takeguchi;T. Furuto;D. Sugimori;I. Okura
发孢甲基弯菌（Methylosinus trichosporium）OB 3b是一种甲烷氧化细菌，含有甲烷单加氧酶，催化甲烷羟基化为甲醇。当甲烷被氧化时，产物随后被同一细菌中所含的甲醇脱氢酶氧化。为了防止甲醇的进一步氧化，细胞悬浮液用甲醇脱氢酶的不可逆抑制剂环丙醇处理，导致细胞外甲醇积累。然而，反应在约3小时终止，最终甲醇浓度低于2.96 mmol/g干电池。甲醇生产效率（生产的甲醇/甲烷消耗的比率）为2.90%。通过选择培养条件和反应条件，反应持续100小时，导致甲醇浓度为152 mmol/g干细胞。该水平比常规反应高51倍，甲醇生产效率为61%。
Methylosinus trichosporium OB3b is a methanotrophic bacterium containing methane mono-oxygenase, catalyzing hydroxylation of methane to methanol. When methane is oxidized, the product is subsequently oxidized by methanol dehydrogenase contained in the same bacterium. To prevent further oxidation of methanol, the cell suspension was treated by cyclopropanol, an irreversible inhibitor for methanol dehydrogenase, leading to extracellular methanol accumulation. However, the reaction was terminated at approx 3 h with a final methanol concentration below 2.96 mmol/g dry cell. The methanol production efficiency (the ratio of the produced methanol per methane consumption) was 2.90%. By selecting the culture conditions and the reaction conditions, the reaction continued for 100 h, resulting in a methanol concentration of 152 mmol/g dry cell. This level was 51 times higher than that of the conventional reaction, and the methanol production efficiency was 61%.
影响因子：
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作者：
Brian G. Fox;Wayne Afroland;David R. Jollie;J. Lipscomb
通讯作者：
Brian G. Fox;Wayne Afroland;David R. Jollie;J. Lipscomb