Applications of enzymes in the synthesis and hydrolytic study of 2-deoxy-α-D-glucopyranosyl phosphate
Applications of enzymes in the synthesis and hydrolytic study of 2-deoxy-α-D-glucopyranosyl phosphate
复制标题
酶在2-脱氧-α-D-吡喃葡萄糖基磷酸合成及水解研究中的应用
DOI：
10.1139/v88-317
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发表时间：
1988
影响因子：
1.1
通讯作者：
S. Withers
中科院分区：
文献类型：
--
作者：
M. Percival;S. Withers
不稳定的磷酸糖 2-脱氧-α-D-吡喃葡萄糖基磷酸酯是通过两步酶法从 2-脱氧-D-葡萄糖-6-磷酸通过中间体尿苷 5'-二磷酸-2-脱氧-D-葡萄糖合成的。通过使用酶测定法测量剩余底物来确定几个温度下酸催化 (1 M HClO4) 水解的速率常数。获得的值与基于烷基-和芳基-2-脱氧-D-吡喃葡萄糖苷的已知水解速率所预期的值一致。
The labile sugar phosphate 2-deoxy-α-D-glucopyranosyl phosphate has been synthesized enzymically in a two-step process from 2-deoxy-D-glucose-6-phosphate via an intermediate uridine 5′-diphospho-2-deoxy-D-glucose. Rate constants for acid-catalysed (1 M HClO4) hydrolysis at several temperatures were determined by using an enzymic assay to measure remaining substrate. The values obtained were consistent with that anticipated on the basis of known hydrolysis rates for alkyl- and aryl-2-deoxy-D-glucopyranosides.