Ion transport in normal and inflamed human jejunum in vitro. Changes with electric field stimulation and theophylline.
Ion transport in normal and inflamed human jejunum in vitro. Changes with electric field stimulation and theophylline.
复制标题
体外正常和发炎的人空肠中的离子转运。
DOI：
10.1007/bf01536793
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发表时间：
1990
影响因子：
3.1
通讯作者：
Renquist,KS
中科院分区：
文献类型：
--
作者：
Hubel,KA;Renquist,KS
我们研究了基础条件下以及组织电刺激（EFS）和5 mM茶碱（N=12对）时人空肠粘膜中的离子转运。将组织固定在通量室中，以测量22 Na、36 Cl的单向通量、短路电流Isc（μeq/cm 2/hr）、电势差PD（mV）和总离子电导G（mS/cm 2）。6对正常或炎症较轻的组织对茶碱的反应是增加PD和Iscand减少JCl（ms），即粘膜至粘膜的Cl流量。在对茶碱或EFS无反应的6对中，炎症更强烈，并且在基础状态下，G更低，PD更高，Na和Cl的单向通量减半。我们得出结论：（1）正常空肠分泌Cl并通过减少JCl（ms）从而增加Cl分泌来响应茶碱或EFS;（2）与人回肠不同，茶碱和EFS都不影响JCl（sm）;（3）炎症消除对茶碱和EFS的响应。
We studied ion transport in human jejunal mucosa under basal conditions and when tissues were stimulated electrically (EFS) and with theophylline 5 mM (N=12 pairs). Tissues were mounted in a flux chamber to measure unidirectional fluxes of22Na,36Cl, shortcircuit current, Isc(μeq/cm2/hr), electrical potential difference, PD (mV), and total ionic conductance, G (mS/cm2). Six pairs of tissues that were normal or less inflamed responded to theophylline by increasing PD and Iscand by reducing JCl(ms), the mucosal to serosal flux of Cl. In six pairs that did not respond to theophylline or to EFS, inflammation was more intense, and in the basal state, G was lower, PD was higher, and unidirectional fluxes of Na and Cl were halved. We conclude: (1) normal jejunum secretes Cl and responds to theophylline or EFS by reducing JCl(ms)thereby increasing Cl secretion; (2) unlike human ileum, neither theophylline nor EFS affect JCl(sm); (3) inflammation eliminates the response to theophylline and to EFS.