The vagal nerve as a link between the nervous and immune system in the instance of polymicrobial sepsis
The vagal nerve as a link between the nervous and immune system in the instance of polymicrobial sepsis
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DOI：
10.1007/s00423-006-0031-y
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发表时间：
2006-04-01
影响因子：
2.3
通讯作者：
Heidecke, CD
中科院分区：
文献类型：
--
作者：
Kessler, W;Traeger, T;Heidecke, CD
背景：迷走神经在自主神经系统中的作用是众所周知的。最近，一个额外的功能被发现作为神经和免疫系统之间的连接器。这种联系被称为“胆碱能炎症通路。通过刺激位于巨噬细胞上的乙酰胆碱受体，可以直接影响“非特异性”免疫系统。方法：在迷走神经穿过膈肌的正后方完全切断迷走神经。使用多微生物腹膜炎（升结肠支架腹膜炎，CASP）的小鼠模型分析完全迷走神经切断术的效果。在CASP模型中分析迷走神经切断或假手术小鼠的存活和临床过程。结果：CASP手术后，迷走神经切断术导致死亡率（64.7%）显著高于假迷走神经切断术动物（34%）。在有或没有迷走神经切断术的脓毒症动物的各种组织（肺、肝、脾、血液、灌洗液和肾）中未观察到细菌负荷的差异。迷走神经切断的动物显示血清细胞因子水平升高（TNF，IL-6，IL-10和MCP-1）后20小时诱导多微生物腹膜炎。结论：迷走神经是免疫系统的重要调节因子。
Background: The role of the vagal nerve in the autonomic nervous system is widely well known. Recently, an additional function was revealed serving as a connector between the nervous and immune system. This connection is called the "cholinergic inflammatory pathway." Through stimulation of the acetylcholine receptors located upon the macrophages, the "unspecific" immune system can be directly influenced. Methods: The vagal nerve was completely transected directly posterior to its passage through the diaphragm. The effect of complete vagotomy was analyzed using a murine model of polymicrobial peritonitis (colon ascendens stent peritonitis, CASP). Survival and clinical course of vagotomized or sham-operated mice were analyzed in the CASP model. Results: After CASP surgery, vagotomy led to a significantly increased mortality (64.7%) in comparison to sham-vagotomized animals (34%). No difference in the bacterial load of various tissues (lung, liver, spleen, blood, lavage fluid, and kidney) from septic animals with or without vagotomy was observed. Vagotomized animals reveal elevated serum cytokine levels (TNF, IL-6, IL-10, and MCP-1) 20 h after the induction of polymicrobial peritonitis. Conclusion: The vagal nerve is therefore an important modulator of the immune system.