Surface-immobilized dextran limits cell adhesion and spreading
Surface-immobilized dextran limits cell adhesion and spreading
复制标题
DOI：
10.1016/s0142-9612(00)00151-4
复制
发表时间：
2000-11-01
期刊：
影响因子：
14
通讯作者：
Letbetter, DS
中科院分区：
文献类型：
--
作者：
Massia, SP;Stark, J;Letbetter, DS
最近，葡聚糖被用作聚乙二醇(PEG)的替代品，用于生物材料表面的低蛋白结合、抗细胞涂层。尽管表面接枝的葡聚糖和聚乙二醇的防污性能非常相似，但当需要将生物活性分子高密度地表面固定到低蛋白质结合的表面涂层上时，葡聚糖的多价性是有利的。用于生物材料应用的葡聚糖固定化的首选方法应该是简单且毒性最小的。在本报告中，描述了一种在温和的水反应条件下使用低残留毒性试剂将葡聚糖共价固定到材料表面的方法。将70 kDa的葡聚糖固定在玻璃和聚对苯二甲酸乙二醇酯(PET)表面。比较3T3成纤维细胞在未处理材料、氨化材料和葡聚糖包被材料上的粘附性。在血清携带的细胞黏附蛋白存在的情况下，葡聚糖涂层有效地限制了细胞在玻璃和PET表面的黏附和扩散。有了葡聚糖为基础的表面涂层，就有可能开发出定义明确的表面修饰，以促进特定的细胞相互作用，并可能在长期生物材料植入中获得更好的性能。(C)2000爱思唯尔科学有限公司。保留所有权利。
Dextran has recently been investigated as an alternative to polyethylene glycol (PEG) for low protein-binding, cell-resistant coatings on biomaterial surfaces. Although anti-fouling properties of surface-grafted dextran and PEG are quite similar, the multivalent properties of dextran are advantageous when high-density surface immobilization of biologically active molecules to low protein-binding surface coatings is desired. The preferred methods of dextran immobilization for biomaterial applications should be simple with minimal toxicity. In this report, a method is described for covalent immobilization of dextran to material surfaces which involves low residual toxicity reagents in mild aqueous reaction conditions. 70kDa MW dextran was immobilized on glass and polyethylene terephthalate (PET) surfaces. 3T3 fibroblast cell adhesion was compared on untreated, aminated, and dextran-coated materials. Dextran coatings effectively limited cell adhesion and spreading on glass and PET surfaces in the presence of serum-borne cell adhesion proteins. With dextran-based surface coatings, it will be possible to develop well-defined surface modifications that promote specific cell interactions and perhaps better performance in long-term biomaterial implants. (C) 2000 Elsevier Science Ltd. All rights reserved.