In vivo imaging of Ras protein's activity in olfactory neurons
In vivo imaging of Ras protein's activity in olfactory neurons
复制标题
嗅觉神经元 Ras 蛋白活性的体内成像
DOI：
10.1038/protex.2013.011
复制
发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Takeshi Ishihara & Takaaki Hirotsu
中科院分区：
文献类型：
--
作者：
Takayuki Uozumi;Ryuji Yamada;Akiya Suzuki;Gun Taniguchi;Kazushi Yoshida;Yuichi Iino;Takeshi Ishihara & Takaaki Hirotsu
信号转导途径在适应环境变化中发挥着重要作用，由各种信号蛋白组成。因此，观察体内特定蛋白质的活性对于理解信号转导途径非常重要。然而，活体动物中信号蛋白的时间动态尚不清楚。在这里，我们试图监测 Ras 蛋白的活性，Ras 蛋白是 Ras-MAPK 信号转导途径的关键调节因子之一。此前有报道称，Ras-MAPK 通路在秀丽隐杆线虫的嗅觉神经元中发挥作用。因此，我们使用 Raichu-Ras（一种基于 FRET 的 Ras 活性传感器）观察了活体动物嗅觉神经元中的 Ras 活性。我们成功地检测到 Ras 活性响应气味刺激的快速变化。
Signal transduction pathways play essential roles to adapt environmental changes and composed of various signaling proteins. Therefore, observation of activity of a specific protein in vivo is important for understanding the signal transduction pathways. However temporal dynamics of signaling proteins in living animals are not well known. Here we tried to monitor the activities of Ras protein which is one of the key regulators for the Ras-MAPK signal transduction pathway. It was previously reported that the Ras-MAPK pathway functions in olfactory neurons in Caenorhabditis elegans. Thus, we observed Ras activity in olfactory neuron in living animals using Raichu-Ras, a FRET-based sensor for Ras activity. We succeeded in detecting quick changes of Ras activity in response to odor stimuli.