The tumour-suppressor genes lgl and dlg regulate basal protein targeting in Drosophila neuroblasts
The tumour-suppressor genes lgl and dlg regulate basal protein targeting in Drosophila neuroblasts
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DOI：
10.1038/35046094
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发表时间：
2000-11-30
期刊：
影响因子：
64.8
通讯作者：
Doe, CQ
中科院分区：
文献类型：
--
作者：
Peng, CY;Manning, L;Doe, CQ
果蝇神经母细胞是研究不对称细胞分裂的模型系统：它们不均匀地分裂产生顶端神经母细胞和基底神经节母细胞，它们在大小、有丝分裂活性和发育潜力上不同。在成神经细胞有丝分裂过程中，顶端蛋白复合物定向有丝分裂纺锤体，并将细胞命运的决定因素靶向基底皮质（1），但每个过程的机制尚不清楚。在这里，我们表明，肿瘤抑制基因致死巨幼虫（lgl）和光盘大（dlg）调节基础蛋白质靶向，但不是顶端复合物的形成或纺锤体的方向，在胚胎和幼虫神经母细胞。Dlg蛋白是顶端富集的，并且是维持Lgl蛋白的皮质定位所需的。基础蛋白靶向需要微丝和肌球蛋白功能，但通过降低肌球蛋白II的水平强烈抑制lgl表型。我们的结论是，DLG和LGL促进，肌球蛋白II抑制，肌动球蛋白依赖的基础蛋白靶向成神经细胞。
Drosophila neuroblasts are a model system for studying asymmetric cell division: they divide unequally to produce an apical neuroblast and a basal ganglion mother cell that differ in size, mitotic activity and developmental potential. During neuroblast mitosis, an apical protein complex orients the mitotic spindle and targets determinants of cell fate to the basal cortex(1), but the mechanism of each process is unknown. Here we show that the tumour-suppressor genes lethal giant larvae (lgl) and discs large (dlg) regulate basal protein targeting, but not apical complex formation or spindle orientation, in both embryonic and larval neuroblasts. Dlg protein is apically enriched and is required for maintaining cortical localization of Lgl protein. Basal protein targeting requires microfilament and myosin function, yet the lgl phenotype is strongly suppressed by reducing levels of myosin II. We conclude that Dlg and Lgl promote, and myosin II inhibits, actomyosin-dependent basal protein targeting in neuroblasts.