ELECTRON TRANSPORT-LINKED NITROUS-OXIDE SYNTHESIS AND REDUCTION BY PARACOCCUS-DENITRIFICANS MONITORED WITH AN ELECTRODE
ELECTRON TRANSPORT-LINKED NITROUS-OXIDE SYNTHESIS AND REDUCTION BY PARACOCCUS-DENITRIFICANS MONITORED WITH AN ELECTRODE
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DOI：
10.1016/0006-291x(82)91370-5
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发表时间：
1982-01-01
影响因子：
3.1
通讯作者：
FERGUSON, SJ
中科院分区：
文献类型：
--
作者：
ALEFOUNDER, PR;FERGUSON, SJ
使用电化学方法测定 N2O 可以方便地测量脱氮假单胞菌的一氧化二氮还原酶活性。该程序的引入证明了 N2O 还原酶活性可逆地受到氧气的抑制；抗霉素强烈抑制电子流向 N2O，并且这种抑制可以被四甲基对苯二胺绕过；抗坏血酸加上四甲基对苯二胺（大概是通过向细胞色素 c 提供电子）是一氧化二氮还原酶的有效还原剂。在一氧化二氮还原酶抑制剂乙炔的存在下，亚硝酸盐迅速产生N2O，这与亚硝酸盐还原酶的产物N2O一致。
The nitrous oxide reductase activity of P. denitrificans can be conveniently measured using an electrochemical method for determining N2O. Introduction of this procedure allowed demonstration that N2O reductase activity is reversibly inhibited by oxygen; antimycin strongly inhibits electron flow to N2O and that this inhibition is bypassed by tetramethyl-p-phenylenediamine; and that ascorbate plus tetramethyl-p-phenylenediamine, presumably by donating electrons to cytochrome c, is an effective reductant for nitrous oxide reductase. In the presence of the nitrous oxide reductase inhibitor, acetylene, N2O is promptly produced from nitrite, consistent with the product of nitrite reductase being N2O.