Optimization and multiomic basis of phenyllactic acid overproduction by Lactobacillus plantarum
Optimization and multiomic basis of phenyllactic acid overproduction by Lactobacillus plantarum
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植物乳杆菌过量生产苯乳酸的优化和多组学基础
DOI：
10.1021/acs.jafc.9b07136
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发表时间：
2020
影响因子：
6.1
通讯作者：
Luo Yiyong
中科院分区：
文献类型：
--
作者：
Wu Wenyu;Deng Gang;Liu Chenjian;Gong Xiaowei;Ma Guanghui;Yuan Qingqing;Yang En;Li Xiaoran;Luo Yiyong
本研究旨在探讨植物乳杆菌苯乳酸（PLA）过度积累的调控机制。菌株S1与菌株m -4-3的PLA含量差异最大，为22.42 mg/L。此外，优化实验表明，在最优条件下（样品m -4-3y） PLA的产量可达400.74 mg/L，是样品m -4-3和S1的7.61 ~ 13.26倍。随后，基因组学、转录组学和代谢组学数据的综合分析显示，与S1相比，m -4-3和m -4-3y虽然缺乏完整的从头合成途径，但通过加强核心途径和中心碳代谢，减弱氨基酸及其衍生物的生物合成途径，增加了PLA的产生。这些变化可以提供足够的前体，并补偿或平衡强化核心途径所消耗的能量。
The goal of this study was to explore the regulatory mechanisms of phenyllactic acid (PLA) overaccumulation inLactobacillus plantarum. The dynamics of PLA production revealed that 24 h was a suitable fermentation time, at which one of the largest differences in PLA content between strains S1 and YM-4-3 was 22.42 mg/L. Additionally, an optimization experiment showed that PLA production under the optimal condition (sample YM-4-3y) was up to 400.74 mg/L, 7.61–13.26 times as those of YM-4-3 and S1. Subsequently, an integrated analysis of genomic, transcriptomic and metabolomic data revealed that, YM-4-3 and YM-4-3y, compared with S1, although lacking a complete de novo biosynthetic pathway, increased PLA production by strengthening the core pathway and central carbon metabolism, and weakening the biosynthesis pathway of amino acids and their derivatives. These changes can provide sufficient precursors and compensate for or balance the energy consumed by the reinforced core pathway.