THE EFFECTS OF EMBEDDING IN WATER-SOLUBLE PLASTICS ON THE FINAL DIMENSIONS OF LIVER SECTIONS
THE EFFECTS OF EMBEDDING IN WATER-SOLUBLE PLASTICS ON THE FINAL DIMENSIONS OF LIVER SECTIONS
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DOI：
10.1111/j.1365-2818.1983.tb04233.x
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发表时间：
1983-01-01
影响因子：
2
通讯作者：
GERRITS, PO
中科院分区：
文献类型：
--
作者：
HANSTEDE, JG;GERRITS, PO
描述了猪肝样品在固定、脱水、渗透和包埋在不同水溶性塑料混合物中的过程中的形态变化。使用的塑料是：3种不同的乙二醇甲基丙烯酸酯（GMA）和市售材料JB 4的混合物。缓冲甲醛固定并没有产生显着的形态学变化的肝脏标本。脱水明显地影响肝脏样本的体积（线性收缩约10%）。9.3%）。脱水之后是渗透阶段。在此阶段，发生轻微溶胀（线性，2-5%）。塑料的最终聚合导致1- 2%的进一步线性收缩。不同的技术因素对纯塑料和塑料包埋肝脏切片的拉伸的影响似乎是相当大的。研究了2-3 μ m切片之间拉伸的差异。注意到温度对截面拉伸的显著影响。所有塑料混合物的切片在293 ℃的温度下拉伸。K显示出比在333 ℃时多3%的线性拉伸。K.讨论了4种塑料混合物之间的差异。在形态学和体视学研究中必须使用校正因子。在将水溶性塑料用作包埋介质时，必须采用标准化程序，特别是在切割和拉伸阶段。
The morphometric changes [pig] liver samples undergo in the course of fixation, dehydration, infiltration and embedding in different mixtures of water-soluble plastics are described. The plastics used were: 3 different mixtures of glycolmethacrylate (GMA) and the commercially available material JB4. Buffered formaldehyde fixation did not produce significant morphometric changes in the liver specimens. Dehydration obviously affects the volume of the liver specimen (linear shrinkage .apprx. 9.3%). The dehydration is followed by an infiltration phase. During this phase a slight swelling (linear, 2-5%) occurs. The final polymerization of the plastic resulted in a further linear shrinkage of 1-2%. The influence of different technical factors on the stretching of sections of pure plastic and of plastic embedded liver appeared to be considerable. The difference in stretching between 2-3 .mu.m sections was studied. A significant influence of temperature upon section stretching was noted. Sections of all plastic mixtures stretched at a temperature of 293.degree. K showed 3% more linear stretch than at a temperature of 333.degree. K. Differences between the 4 plastic mixtures are discussed. Correction factors must be used in morphometrical and stereological investigations. In the application of water-soluble plastics as embedding media it is essential to apply a standardized procedure, particularly in the cuttting and stretching phase.