MUSCARINE AFFECTS CALCIUM-CURRENTS IN RAT HIPPOCAMPAL PYRAMIDAL CELLS-INVITRO
MUSCARINE AFFECTS CALCIUM-CURRENTS IN RAT HIPPOCAMPAL PYRAMIDAL CELLS-INVITRO
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DOI：
10.1016/0304-3940(87)90419-8
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发表时间：
1987-05-19
影响因子：
2.5
通讯作者：
BROWN, DA
中科院分区：
文献类型：
--
作者：
GAHWILER, BH;BROWN, DA
在海马片培养的单个CA3锥体细胞上记录到钙电流，通过一个Cs+或K+填充的微电极电压钳制，并用含1微米河豚毒素和10 mM四乙铵的Hanks‘s平衡盐液灌流。灌流10-100µm的毒扁豆碱可使钙电流可逆性降低。这种作用可被哌仑西平(10µm)或阿托品(1µm)抑制。在充满K+的细胞中，抑制之前有一个内向电流增强的阶段；这被认为是毒扁豆碱引起的快速外向电流抑制的次要阶段，因为当外向电流被Ba2+预先抑制时，这种抑制被降低。在部分钳制或非钳制细胞中，抑制钙电流会导致钙尖峰平台缩短。
Ca2+-currents were recorded from single CA3 pyramidal cells in hippocampal slice cultures, voltage-clamped through a single Cs+- or K+-filled microelectrode and perfused with Hanks'' balanced salt solution containing 1 .mu.M tetrodotoxin and 10 mM tetraethylammonium. The Ca2+-current was reversibly reduced by bath-perfused muscarine (10-100 .mu.M). This effect was inhibited by pirenzepine (10 .mu.M) or atropine (1 .mu.M). In K+-filled cells, inhibition was preceded by a phase of inward current enhancement; this was considered to be secondary to rapid outward current inhibition induced by muscarine since it was reduced when outward currents were previously inhibited with Ba2+. In partially clamped or unclamped cells inhibition of Ca2+-current leads to a shortening of the Ca2+-spike plateau.