Overexpression of the endoplasmic reticulum chaperone BiP3 regulates XA21-mediated innate immunity in rice.

Overexpression of the endoplasmic reticulum chaperone BiP3 regulates XA21-mediated innate immunity in rice.
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DOI:
10.1371/journal.pone.0009262
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发表时间:
2010-02-17
期刊:
影响因子:
3.7
通讯作者:
Ronald PC
Ronald PC
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Park CJ;Bart R;Chern M;Canlas PE;Bai W;Ronald PC

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模式识别受体 (PRR) 对病原体相关分子模式的识别会激活先天免疫反应。尽管 PRR 介导的信号事件对于动植物的生存至关重要,但 PRR 的分泌和定位尚未明确阐明。在这里,我们报告了内质网 (ER) 伴侣 BiP3 与水稻 XA21 PRR 的体内相互作用,这赋予了对革兰氏阴性细菌米黄单胞菌 (Xanthomonas oryzae pv.) 的抗性。米曲霉(Xoo)。我们发现 XA21 是糖基化的,主要定位于 ER 和质膜 (PM)。在 BiP3 过表达的水稻植物中,XA21 介导的免疫受到损害,XA21 稳定性显着降低,并且 XA21 蛋白水解裂解受到抑制。 BiP3 过度表达不会影响一般水稻防御反应、细胞死亡或油菜素内酯诱导的反应。这些结果表明 BiP3 调节 X​​A21 蛋白的稳定性和加工,并且这种调节对于 Xoo 的抗性至关重要。
Recognition of pathogen-associated molecular patterns by pattern recognition receptors (PRRs) activates the innate immune response. Although PRR-mediated signaling events are critical to the survival of plants and animals, secretion and localization of PRRs have not yet been clearly elucidated. Here we report the in vivo interaction of the endoplasmic reticulum (ER) chaperone BiP3 with the rice XA21 PRR, which confers resistance to the Gram negative bacterium, Xanthomonas oryzae pv. oryzae (Xoo). We show that XA21 is glycosylated and is primarily localized to the ER and also to the plasma membrane (PM). In BiP3-overexpressing rice plants, XA21-mediated immunity is compromised, XA21 stability is significantly decreased, and XA21 proteolytic cleavage is inhibited. BiP3 overexpression does not affect the general rice defense response, cell death or brassinolide-induced responses. These results indicate that BiP3 regulates XA21 protein stability and processing and that this regulation is critical for resistance to Xoo.
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