Finding and using hyperthermophilic enzymes
Finding and using hyperthermophilic enzymes
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DOI：
10.1016/s0167-7799(98)01193-7
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发表时间：
1998-08-01
影响因子：
17.3
通讯作者：
Kelly, RM
中科院分区：
文献类型：
--
作者：
Adams, MWW;Kelly, RM
最近的发展增强了发现超嗜热酶的前景。这一点很重要，因为超嗜热酶的非凡热稳定性的内在基础尚未被揭示，因此目前不可能将这种特性工程化到不太嗜热的酶中。在嗜温宿主中克隆和表达编码超嗜热酶的基因的成功努力提高了高温生物催化剂的可用性。剩下的任务是确定的机会，使战略利用的热活性和热稳定性的超嗜热酶。
Recent developments have enhanced the prospects for the discovery of hyperthermophilic enzymes. This is important because the intrinsic basis underlying the extraordinary thermostability of hyperthermophilic enzymes has yet to be revealed, and so engineering this characteristic into less thermophilic enzymes is not possible at this time. Successful efforts to clone and express the genes encoding hyperthermophilic enzymes in mesophilic hosts have improved the availability of high-temperature biocatalysts. The remaining task is the identification of opportunities to make strategic use of the thermoactivity and thermostability of hyperthermophilic enzymes.