Temperature measurement of microfluids with high temporal resolution by laser-induced fluorescence
Temperature measurement of microfluids with high temporal resolution by laser-induced fluorescence
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DOI：
10.1007/s12206-009-0609-8
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发表时间：
2009-07-01
影响因子：
1.6
通讯作者：
Honami, Shinji
中科院分区：
文献类型：
--
作者：
Motosuke, Masahiro;Akutsu, Dai;Honami, Shinji
微流控系统由于尺度效应的影响，热传导速度快，热容小，对温度非常敏感。本研究的目的是开发一种具有高空间和时间分辨率的微流控装置中液体温度场的测量系统。本研究所采用的测量方法是利用荧光素的激光诱导荧光，其荧光强度具有温度依赖性。为了测量瞬态温度场，使用图像增强高速摄像机。信噪比可以通过时间或相位平均方案来改善。应用同步机制，相位平均温度数据的时间分辨率为500 μ s。从瑞利极限估计的空间分辨率约为530 nm。通过对微流控装置中激光加热下液体温度瞬态行为的实验，验证了所开发的测量系统的有效性。
Temperature of microfluidic system is greatly sensitive because of fast heat conduction and small heat capacity due to the scale effect. The purpose Of this Study is the development of a measurement system for the temperature field of liquids in a microfluidic device with high spatial- and temporal-resolution. Measurement method employed in this study, is laser-induced fluorescence using fluorescein with the temperature dependence Of fluorescent intensity. In order to measure the transient temperature field, an image-intensified high-speed camera was utilized. The signal-to-noise ratio can be improved by the time- or phase-averaging scheme. Applying the synchronization mechanism, phase-averaged temperature data with the time resolution of 500 mu s can be obtained. Spatial resolution estimated from the Rayleigh limit was approximately 530 nm. The validity of the developed measurement system was confirmed by the experiments for the transient behavior of the liquid temperature undergoing the laser heating in the microfluidic device.