The dermal-epidermal junction of human skin contains a novel laminin variant.
The dermal-epidermal junction of human skin contains a novel laminin variant.
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DOI：
10.1083/jcb.119.3.695
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发表时间：
1992-11
期刊：
影响因子：
--
通讯作者：
Burgeson RE
中科院分区：
文献类型：
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作者：
Marinkovich MP;Lunstrum GP;Keene DR;Burgeson RE
我们报告了一种新的层粘连蛋白变体的鉴定，它似乎是上皮基底膜的一个子集所独有的。该变异体含有两条在电泳学和免疫学上与B1和B2链相同的链。分子中不存在层粘连蛋白A链中包含的表位，而190 kD的链取代了A链。V8蛋白酶分析和Western blotting研究表明，该变异蛋白190-kD链在结构和免疫学上与加里宁的200-kD链具有相似性。旋转跟踪分析表明，190-kD链为变异的长臂贡献了一个大的球状结构，但缺乏A链贡献给层粘连蛋白的短臂。该变异体是由培养的皮肤外植体、人类角质形成细胞和一种鳞状细胞癌细胞产生的，存在于人羊水中。针对最近表征的一种新的锚定细丝成分加里宁的多克隆抗体和识别加里宁的一个亚单位的单抗BM165(Rousselle等人，1991)在非还原条件下与变异体发生交叉反应。使用交叉反应的抗Kalinin抗血清对人体组织进行的免疫组织学研究表明，这种层粘连蛋白变体的分布至少限于含有基底膜的锚定细丝。我们建议将该变体命名为K-层粘连蛋白。
We report the identification of a novel laminin variant that appears to be unique to a subset of epithelial basement membranes. The variant contains two chains electrophoretically and immunologically identical to the B1 and B2 chains. Epitopes contained in the laminin A chain are absent from the molecule, and a 190-kD chain substitutes for the A chain. V8 protease analysis and Western blotting studies indicate that the variant 190-kD chain shows structural and immunological similarity to the 200-kD chain of kalinin. Rotary shadowing analysis indicates that the 190-kD chain contributes a large globular structure to the variant long arm, but lacks the short arm contributed to laminin by the A chain. The variant is produced by cultured skin explants, human keratinocytes and a squamous cell carcinoma line, and is present in human amniotic fluid. Polyclonal antibodies raised to kalinin, a recently characterized novel component of anchoring filaments, and mAb BM165 which recognizes a subunit of kalinin (Rousselle et al., 1991) cross react with the variant under nonreducing conditions. Immunohistological surveys of human tissues using the crossreacting antikalinin antiserum indicate that the distribution of this laminin variant is at least restricted to anchoring filament containing basement membranes. We propose the name K-laminin for this variant.