Radioimmunoassay of neurotensin and the distribution and concentration of gut neurotensin in rat and dog.
Radioimmunoassay of neurotensin and the distribution and concentration of gut neurotensin in rat and dog.
复制标题
神经降压素的放射免疫测定以及大鼠和狗肠道神经降压素的分布和浓度。
DOI：
10.1620/tjem.130.129
复制
发表时间：
1980
期刊：
影响因子：
--
通讯作者：
A. Shibata
中科院分区：
文献类型：
--
作者：
Y. Iwasaki;S. Ito;A. Shibata
建立了神经降压素(NT)的放射免疫分析方法。用人工合成的NT十三肽与牛血清白蛋白通过戊二醛偶联免疫4只兔，获得4份高亲和力的抗血清。用改良的Hunter-Greenwood‘s氯胺-T法用Na125I标记NT。RIA的灵敏度为100pg/ml，抗血清与其他多肽无交叉反应。用放射免疫法测定了胃肠(GI)-NT在大鼠和犬GI组织中的分布和浓度。使用四种抗血清的RIA同样检测到空回肠段的浓度高于其他地区。虽然GI-NT在大鼠和狗体内的分布非常相似，但其浓度存在物种特异性差异。
A specific radioimmunoassay (RIA) for neurotensin (NT) has been developed. Four antisera of high avidity were raised in 4 rabbits immunized with synthetic NT tridecapeptide coupled with bovine serum albumin via glutaraldehyde. NT was labeled with Na125I by means of modified Hunter-Greenwood's chloramine-T method. Sensitivity of the RIA was 100 pg/ml. Cross-reactivity of antiserum to other peptides was not observed. Using the RIA, the distribution and concentration of gastrointestinal (GI)-NT have been determined in acid-acetone extracts of rat and dog GI tissues. RIA using four kinds of antisera similarly detected higher concentrations in jejuno-ileal sections than in other regions. Although the distribution of GI-NT was quite similar between rat and dog, its concentration showed species specific difference.