Linkage map construction and mapping QTL for cotton fibre quality using SRAP, SSR and RAPD
Linkage map construction and mapping QTL for cotton fibre quality using SRAP, SSR and RAPD
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DOI：
10.1111/j.1439-0523.2004.01039.x
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发表时间：
2005-04-01
期刊：
影响因子：
2
通讯作者：
Stewart, JM
中科院分区：
文献类型：
--
作者：
Lin, Z;He, D;Stewart, JM
四倍体棉花是种植最广泛的棉花品种之一。两种四倍体品种，绵棉（Gossypium hirsutum L.）和巴氏棉（g.b arbadense L.），主导着世界棉花生产。为了更好地了解棉纤维性状的遗传基础，为提高棉纤维品质，利用序列相关扩增多态性（srap）、简单序列重复（SSRs）和随机扩增多态性dna （RAPDs）构建了四倍体棉花的遗传连锁图谱。利用238对SRAP引物组合、368对SSR引物组合和600对RAPD引物对棉花种质间的多态性进行筛选。Handan208和G. barbadense cv。共发现749个多态性位点（其中SSRs 205个，rapd 107个，srap 437个）。用749个多态性标记对‘Handan208’与‘Pima90’种间杂交的69个F-2后代进行了基因分型。共有566个位点被组装成41个连锁组，每组至少有3个位点。利用已知染色体位置的SSR标记将28个连锁群分配到相应的染色体上。地图面积为5141.8 cM，平均位点间距为9.08 cM。chi(2)检验与预期比例（1:2:1或3:1）偏差的显著性，发现135个位点（18.0%）存在偏隔离，其中大多数存在母亲本等位基因过剩。共检测到13个与纤维性状相关的QTL，其中与纤维强度有关的QTL 2个，与纤维长度有关的QTL 4个，与马克隆值有关的QTL 7个。这些QTL分布在9个连锁群上，解释了16.18 ~ 28.92%的性状变异。6个QTL位于A亚基因组，6个QTL位于D亚基因组，1个QTL位于未分配连锁组。有3个麦克隆值QTL聚集在LG1上，这对分子标记辅助选择改良该性状非常有用。
Tetraploid cotton is one of the most extensively cultivated species. Two tetraploid species, Gossypium hirsutum L. and G. barbadense L., dominate the world's cotton production. To better understand the genetic basis of cotton fibre traits for the improvement of fibre quality, a genetic linkage map of tetraploid cotton was constructed using sequence-related amplified polymorphisms (SRAPs), simple sequence repeats (SSRs) and random amplified polymorphic DNAs (RAPDs). A total of 238 SRAP primer combinations, 368 SSR primer pairs and 600 RAPD primers were used to screen polymorphisms between G. hirsutum cv. Handan208 and G. barbadense cv. Pima90 which revealed 749 polymorphic loci in total (205 SSRs, 107 RAPDs and 437 SRAPs). Sixty-nine F-2 progeny from the interspecific cross of 'Handan208' x 'Pima90' were genotyped with the 749 polymorphic markers. A total of 566 loci were assembled into 41 linkage groups with at least three loci in each group. Twenty-eight linkage groups were assigned to corresponding chromosomes by SSR markers with known chromosome locations. The map covered 5141.8 cM with a mean interlocus space of 9.08 cM. A chi(2) test for significance of deviations from the expected ratio (1 : 2 : 1 or 3 : 1) identified 135 loci (18.0%) with skewed segregation, most of which had an excess of maternal parental alleles. In total, 13 QTL associated with fibre traits were detected, among which two QTL were for fibre strength, four for fibre length and seven for micronaire value. These QTL were on nine linkage groups explaining 16.18-28.92% of the trait variation. Six QTL were located in the A subgenome, six QTL in the D subgenome and one QTL in an unassigned linkage group. There were three QTL for micronaire value clustered on LG1, which would be very useful for improving this trait by molecular marker-assisted selection.