Deletions in the repeating sequences of the toxin A gene of toxin A-negative, toxin B-positive Clostridium difficile strains
Deletions in the repeating sequences of the toxin A gene of toxin A-negative, toxin B-positive Clostridium difficile strains
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DOI：
10.1016/s0378-1097(99)00196-2
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发表时间：
1999-06-15
影响因子：
2.1
通讯作者：
Lyerly, DM
中科院分区：
文献类型：
--
作者：
Kato, H;Kato, N;Lyerly, DM
确定了艰难梭菌毒素 A 阴性、毒素 B 阳性（毒素 A-、毒素 B+）菌株的毒素 A 基因的重复序列，这些菌株是在日本和印度尼西亚地理上分开的设施中分离的。所测试的所有六种菌株均具有相同的重复序列，但毒素 A 基因中有两个缺失（大小为 1548 个和 273 个核苷酸）。设计了PCR方法来检测缺失，并在通过该方法检查的所有50个毒素A-、毒素B+菌株中证实了缺失。 Western免疫印迹分析表明，针对天然毒素A的多克隆抗血清不与浓缩物发生反应。毒素A-、毒素B+菌株的培养滤液。这些结果可能表明毒素A-、毒素B+菌株删除了毒素A基因的三分之二的重复区，该基因编码完全负责与多克隆抗血清反应的表位。 (C) 1999 年欧洲微生物学会联合会。由 Elsevier Science B.V. 出版。保留所有权利。
The repeating sequences of the toxin A gene from toxin A-negative, toxin B-positive (toxin A-, toxin B+) strains of Clostridium,difficile which were isolated in geographically separated facilities in Japan and Indonesia were determined. All six strains tested had identical repeating sequences with two deletions (1548 and 273 nucleotides in size) in the toxin A gene. A PCR method was designed to detect the deletions and the deletions were confirmed in all 50 toxin A-, toxin B+ strains examined by this method. Western immunoblot analysis revealed that polyclonal antiserum against native toxin A did not react with the concentrated. culture filtrates of the toxin A-, toxin B+ strains. These resulrs may suggest that toxin A-, toxin B+ strains have deletions of the two thirds of the repeating regions of the toxin A gene, which encodes the epitopes fully responsible for the reaction with the polyclonal antiserum. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.