Genome-wide identification and expression analysis of stress-associated proteins (SAPs) containing A20/AN1 zinc finger in cotton
Genome-wide identification and expression analysis of stress-associated proteins (SAPs) containing A20/AN1 zinc finger in cotton
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DOI：
10.1007/s00438-016-1252-6
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发表时间：
2016-12-01
影响因子：
3.1
通讯作者：
Song, Chunpeng
中科院分区：
文献类型：
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作者：
Gao, Wei;Long, Lu;Song, Chunpeng
含有A20/AN 1锌指结构域的逆境相关蛋白（SAP）在植物对生物和非生物逆境的应答中起着重要作用。然而，很少有研究集中在棉花SAP基因家族。为了探索这些基因的分布和表达模式，我们在四倍体陆地棉中进行了SAP的全基因组鉴定和表征。TM-1（AD 1）。共鉴定出37个编码SAP的基因，其中36个在A和D亚基因组中重复。基因结构和保守蛋白基序分析表明，几乎所有的A20-AN 1型SAP都是无内含子的，而AN 1-AN 1型SAP含有一个内含子。研究了SAP启动子的顺式元件，以及基于RNA-seq数据并通过qRT-PCR验证的棉花SAP基因在不同胁迫下的表达水平。多数棉花SAP基因是由多种逆境和激素诱导的，特别是盐胁迫，表明SAP基因可能在棉花应对不利环境变化中发挥重要作用。在这些鉴定的SAP中，GhSAP 17 A/D的表达在棉花对大丽轮枝菌的反应中被抑制，并且GhSAP 17 A/D沉默的棉花对大丽轮枝菌表现出更强的抗性。本研究提供了深入了解SAP基因在高地棉的进化，并可能有助于进一步的A20/AN 1型蛋白的功能鉴定和棉花对不同胁迫的反应。
Stress-associated proteins (SAPs) containing the A20/AN1 zinc-finger domain play important roles in response to both biotic and abiotic stresses in plants. Nevertheless, few studies have focused on the SAP gene family in cotton. To explore the distributions and expression patterns of these genes, we performed genome-wide identification and characterization of SAPs in tetraploid Gossypium hirsutum L. TM-1 (AD1). A total of 37 genes encoding SAPs were identified, 36 of which were duplicated in the A and D sub-genomes. The analysis of gene architectures and conserved protein motifs revealed that nearly all A20-AN1-type SAPs were intron-free, whereas AN1-AN1-type SAPs contained one intron. The cis-elements of the SAP promoters were studied, as were the expression levels of cotton SAP genes under different stresses based on RNA-seq data and validated by qRT-PCR. Most cotton SAP genes were induced by multiple stresses and phytohormones, particularly salt stress, indicating that SAP genes may play important roles in cotton's response to unfavorable environmental changes. Among these identified SAPs, the expression of GhSAP17A/D is suppressed in cotton response to Vertillium dahliae, and the GhSAP17A/D-silenced cotton exhibits more resistance to V. dahliae. This study provides insight into the evolution of SAP genes in upland cotton and may aid in efforts at further functional identification of A20/AN1-type proteins and cotton's response to different stresses.