Purified human factor activates heat shock promoter in a HeLa cell-free transcription system.
Purified human factor activates heat shock promoter in a HeLa cell-free transcription system.
复制标题
DOI：
--
复制
发表时间：
1988-12
期刊：
影响因子：
--
通讯作者：
Carlos J. Goldenberg;Yan Luob;Mary Fennap;Ruben Baler;Roberto Weinmannll;Richard Voellmyt
中科院分区：
文献类型：
--
作者：
Carlos J. Goldenberg;Yan Luob;Mary Fennap;Ruben Baler;Roberto Weinmannll;Richard Voellmyt
热休克蛋白（hsp）基因通常是沉默的，并被包括热在内的各种应激激活。作为理解这种激活事件的第一步，已经通过序列特异性DNA亲和色谱法从热处理的HeLa细胞提取物中纯化了约14，000倍的人因子，在此称为人热休克转录因子（人HTF）。如足迹法实验所示，人HTF的最高度纯化的部分特异性地结合到已知的hsp基因的调节序列元件（HSE）。纯化的人HTF具有83 kDa的表观分子量。人HTF是激活来自人细胞的重建体外转录系统中的hsp基因启动子所特别需要的。激活依赖于转录模板中存在的HSE。
Heat shock protein (hsp) genes are typically silent and are activated by various stresses including heat. As a first step toward understanding this activation event, a human factor, referred to here as human heat shock transcription factor (human HTF), has been purified approximately 14,000-fold from extracts of heat-treated HeLa cells by means of sequence-specific DNA affinity chromatography. The most highly purified fraction of human HTF binds specifically to the known regulatory sequence element (HSE) of hsp genes as shown by footprinting experiments. Purified human HTF has an apparent molecular mass of 83 kDa. Human HTF is specifically required for activation of an hsp gene promoter in a reconstituted in vitro transcription system from human cells. Activation is dependent on the presence of the HSEs in the transcription template.