Distribution and molecular characteristics of Vibrio cholerae O1 El Tor isolates recovered in Guangdong Province, China, 1961-2013.
Distribution and molecular characteristics of Vibrio cholerae O1 El Tor isolates recovered in Guangdong Province, China, 1961-2013.
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DOI：
10.1016/j.meegid.2015.11.004
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发表时间：
2016
期刊：
影响因子：
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通讯作者：
Baisheng Li;Rongfeng Chen;Duochun Wang;H. Tan;B. Ke;D. He;C. Ke;Yonghui Zhang
中科院分区：
文献类型：
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作者：
Baisheng Li;Rongfeng Chen;Duochun Wang;H. Tan;B. Ke;D. He;C. Ke;Yonghui Zhang
中国广东省与印度加尔各答和孟加拉国达卡位于同一纬度，也被认为是流行性霍乱的来源地。然而，广东霍乱弧菌O1 El Tor分离株之间的分子描述和遗传关系仍不清楚。在这项研究中，381 个临床V。通过 PCR、扩增子测序和脉冲场凝胶电泳 (PFGE) 对 1961 年至 2013 年间广东霍乱病例中回收的霍乱 O1 菌株进行了研究。在此时间范围内，根据导致流行的不同优势血清型，观察到了四个不同的流行时期（1-4），对应于年份；或时间段分别为1961-1969、1978-1989、1990-2000、2001-2013。对代表性分离株的分子分析表明，单个主导克隆与每个流行阶段相关。第 1 期和第 2 期的所有分离株均携带典型的 El TorctxB；该等位基因从 1993 年开始被 classicctxB 取代。然而，所有分离株均携带 El Tor 特异性毒素共调节菌毛亚基 A (tcpA)。根据NotI酶消化的PFGE将分离株分为五个簇，前四个簇与特定时期相关，分别为簇I（时期1）、II（时期3）、III（时期2）和IV（时期4）。虽然第五簇由所有四个流行时期的分离株组成，但外观上最为异质。我们的数据表明遗传变异影响了新出现的弧菌分离株之间的关系。广东省的霍乱O1导致了第七次全球大流行。
China's Guangdong Province is located along the same latitude as Kolkata, India and Dhaka, Bangladesh, and is also considered a source of epidemic cholera. However, molecular description and the genetic relationships betweenVibrio choleraeO1 El Tor isolates in Guangdong remain unclear. In this study, 381 clinicalV. choleraeO1 isolates recovered from cholera cases presenting in Guangdong between 1961 and 2013 were investigated by PCR, amplicon sequencing and pulsed-field gel electrophoresis (PFGE). During this time frame, four distinct epidemic periods (1–4) were observed based on the different dominant serotype leading its epidemic, correspond to years; or time periods from/to 1961–1969, 1978–1989, 1990–2000, 2001–2013, respectively. Molecular analysis of representative isolates indicated that a single dominating clone was associated with each epidemic stage. All isolates from periods 1 and 2 carried the typical El TorctxB; this allele was displaced by classicalctxBbeginning in 1993. However all isolates carried the El Tor-specific toxin-coregulated pili subunit A (tcpA). Isolates were grouped into five clusters on the basis ofNotI enzyme digested PFGE, and the first four clusters were associated with specific periods, cluster I (period 1), II (period 3), III (period 2) and IV (period 4), respectively. While cluster V consisted of isolates from all four epidemic periods, but was most heterogeneous in appearance. Our data indicate genetic variations that shape the relationship among emerging isolates ofV. choleraeO1 in Guangdong Province contribute to the 7th global pandemic.