CHRONIC CHANGES IN RESPONSE OF CELLS IN ADULT CAT DORSAL HORN FOLLOWING PARTIAL DEAFFERENTATION - APPEARANCE OF RESPONDING CELLS IN A PREVIOUSLY NONRESPONSIVE REGION
CHRONIC CHANGES IN RESPONSE OF CELLS IN ADULT CAT DORSAL HORN FOLLOWING PARTIAL DEAFFERENTATION - APPEARANCE OF RESPONDING CELLS IN A PREVIOUSLY NONRESPONSIVE REGION
复制标题
DOI：
10.1016/0006-8993(76)90899-4
复制
发表时间：
1976-01-01
期刊：
影响因子：
2.9
通讯作者：
WALL, PD
中科院分区：
文献类型：
--
作者：
BASBAUM, AI;WALL, PD
成年猫一侧腰椎膨大的部分传入被切断至L3的所有背根，但S1背根除外。在切断神经根后的不同时间，通过细胞外玻璃微电极记录背角细胞对自然刺激和电刺激小腿和侧翼的反应。当对动物进行部分去传入后24小时的检查时，在L4和L5节段之间对腿部皮肤刺激做出单突触反应的区域内没有细胞。到1wk时，L4-5区开始出现细胞，该区域对外周刺激做出单调反应。这些新连接的细胞数量似乎稳定了1个月。在部分去传入后，这些细胞的特性有6种异常。细胞感受野的位置是S1皮肤节或L4吻侧节段的特征。有些细胞有两个感受野，一个在腿上，一个在腹部。与正常背角相比，感受野的大小变化更大。某些细胞的感受野异常小，边缘陡峭，没有相关的抑制野。这些细胞从高阈值传入的会聚比通常观察到的要少。相关的抑制场很少遇到。观察到习惯化，在一些具有双感受野的细胞中，对一个区域的反应习服，而对另一个区域的反应不受影响。脊髓表面的慢波记录显示，刺激S1皮肤刀的外周效应在脊髓的慢性去传入侧比在正常侧或在正常脊髓中扩散得更广泛。在部分去传入后，剩余的传入细胞可以与去传入细胞建立联系，但所提供的数据不能得出新的联系是通过萌发还是通过揭开现有联系的面纱而产生的结论。
One side of the lumbar enlargement in adult cats was partially deafferented by cutting all dorsal roots caudal to L3 with the exception of the S1 dorsal root. At various times after the roots were sectioned, the response of dorsal horn cells to natural and electrical stimuli applied to the leg and flank were recorded through extracellular glass microelectrodes. When animals were examined up to 24 h after this partial deafferentation, no cells were located in a region between segments L4 and 5 which responded monosynaptically to cutaneous stimulation on the leg. By 1 wk cells began to appear in the L4-5 region which responded monosynaptically to peripheral stimuli. The numbers of these newly connected cells seemed to have stabilized by 1 mo. after the partial deafferentation, but the properties of these cells were abnormal in 6 ways. The location of the receptive field of the cells was characteristic either of the S1 dermatome or of segments rostral to L4. Some cells had double receptive fields, one on the leg and one on the abdomen. The size of the receptive field varied more than observed in normal intact dorsal horn. Certain cells had unusually small receptive fields with abrupt edges and no associated inhibitory fields. The cells received less convergence from high threshold afferents than normally observed. Associated inhibitory fields were rarely encountered. Habituation was observed, and in some cells with double receptive fields the response to one area habituated while the response to the other area was unaffected. Slow wave recording on the surface of the cord showed that the effect of peripheral stimulation of the S1 dermatome spread far more extensively on the chronically deafferented side of the cord than it did on the intact side or in an intact cord. Following partial deafferentation the remaining afferents can establish connection with deafferented cells, but the data presented did not allow a conclusion as to whether the new connections were produced by sprouting or by the unmasking of existing connections.