Hypophysiotropic and neuromodulatory regulation of adrenocorticotropin in the human fetal pituitary gland.
Hypophysiotropic and neuromodulatory regulation of adrenocorticotropin in the human fetal pituitary gland.
复制标题
人胎儿垂体中促肾上腺皮质激素的促垂体和神经调节调节。
DOI：
10.1172/jci112563
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Jaffe,RB
中科院分区：
文献类型：
--
作者：
Blumenfeld,Z;Jaffe,RB
合成人促肾上腺皮质激素释放因子（hCRF）刺激ACTH分泌的人胎儿垂体灌流和分散的人胎儿垂体细胞培养的细胞外基质静态孵育从14至23周胎龄。精氨酸加压素（AVP）可增强hCRF在体外的作用。8-Br-cAMP诱导的反应与hCRF相似。AVP对ACTH的作用与CRF和8-Br-cAMP有协同作用。用地塞米松预处理24小时或同时处理3小时，可非竞争性抑制hCRF介导的ACTH分泌。无论是催产素，儿茶酚胺，肾上腺素，也没有吲哚美辛分泌产生显着的影响，无论是单独或与hCRF或AVP在胎龄研究。这些结果支持CRF在促皮质细胞分泌调节中的生理作用，早在妊娠14周，此时促皮质细胞和分泌ACTH的能力已被证明。
Synthetic human corticotropin-releasing factor (hCRF) stimulated ACTH secretion by human fetal pituitaries in superfusion and dispersed human fetal pituitary cells cultured on an extracellular matrix in static incubation from 14 to 23 wk gestational age. The action of hCRF in vitro was potentiated by arginine vasopressin (AVP) at all ages studied. 8-Br-cAMP induced a response similar to hCRF. The AVP effect on ACTH was synergistic with both CRF and 8-Br-cAMP. hCRF-mediated secretion of ACTH was noncompetitively inhibited by 24-h pretreatment, or by 3-h concomitant treatment, with dexamethasone. Neither oxytocin, catecholamines, prostaglandins, nor indomethacin exerted significant effects on ACTH secretion, either alone or in combination with hCRF or AVP during the gestational ages studied. These results support a physiologic role for CRF in the regulation of secretion by corticotropic cells as early as 14 wk gestation, by which time corticotropes and ability to secrete ACTH have been demonstrated.