Duplication and evolution of devA-like genes in Streptomyces has resulted in distinct developmental roles.
Duplication and evolution of devA-like genes in Streptomyces has resulted in distinct developmental roles.
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DOI：
10.1371/journal.pone.0025049
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Hoskisson PA
中科院分区：
文献类型：
--
作者：
Clark LC;Hoskisson PA
了解形态转变对于阐明许多生物体的进化和发育生物学至关重要。革兰氏阳性土壤细菌天蓝色链霉菌具有复杂的生命周期，非常适合此类研究。我们最近发现了一种转录调节因子 devA，它是该生物体正确形成孢子所必需的，突变体形成短的、错隔的气生菌丝。 devA 及其副本 devE 在链霉菌属中高度保守。 devE 的破坏表明该基因也在孢子形成中发挥作用；然而，devE 突变体的表型与 devA 突变体不同，形成长的无隔膜气生菌丝。 devA和devE的转录分析表明它们在生命周期的不同阶段表达。这表明复制后它们在调节和功能上出现了分歧。对完整测序的放线菌基因组的分析表明，在形态较简单的放线菌中发现了 devA 的单个拷贝，这表明重复导致了形态复杂性的增加。对来自盐孢菌 (Salinispora) 的 devA 的互补研究表明，祖先基因不能与 devA 或 devE 互补，表明新功能化已经发生。对 devA 旁系同源物内同义和非同义核苷酸变化的分析表明，由于两个拷贝都偏离了祖先序列，因此发生了亚功能化。与效应子结合/寡聚化结构域相比，DNA结合结构域中观察到的功能限制水平更高，分歧也是不对称的，这表明这些旁系同源物的底物特异性的多样化促进了它们的进化。
Understanding morphological transformations is essential to elucidating the evolution and developmental biology of many organisms. The Gram-positive soil bacterium, Streptomyces coelicolor has a complex lifecycle which lends itself well to such studies. We recently identified a transcriptional regulator, devA, which is required for correct sporulation in this organism, with mutants forming short, mis-septate aerial hyphae. devA is highly conserved within the Streptomyces genus along with a duplicate copy, devE. Disruption of devE indicates this gene also plays a role in sporulation; however the phenotype of a devE mutant differs from a devA mutant, forming long un-septate aerial hyphae. Transcriptional analysis of devA and devE indicates that they are expressed at different stages of the lifecycle. This suggests that following duplication they have diverged in regulation and function. Analysis of fully sequenced actinomycete genomes shows that devA is found in a single copy in morphologically simpler actinobacteria, suggesting that duplication has lead to increased morphological complexity. Complementation studies with devA from Salinispora, which sporulates but does not form aerial hyphae, indicates the ancestral gene cannot complement devA or devE, suggesting neo-functionalisation has occurred. Analysis of the synonymous and non-synonymous nucleotide changes within the devA paralogues suggest subfunctionalisation has occurred as both copies have diverged from the ancestral sequences. Divergence is also asymmetric with a higher level of functional constraint observed in the DNA binding domain compared with the effector binding/oligomerisation domain, suggesting diversification in the substrate specificity of these paralogues has contributed to their evolution.
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影响因子：
3.4
作者：
Campanaro S;Treu L;Valle G
通讯作者：
Valle G
影响因子：
10.7
作者：
Johnson, Deborah A.;Thomas, Michael A.
通讯作者：
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影响因子：
3.2
作者：
Hillerich, Brandan;Westpheling, Janet
通讯作者：
Westpheling, Janet
DOI：
10.1073/pnas.0337542100
发表时间：
2003-02-18
影响因子：
11.1
作者：
Gust, B;Challis, GL;Chater, KF
通讯作者：
Chater, KF
DOI：
10.1099/00207713-28-2-304
发表时间：
1978-01-01
期刊：
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY
影响因子：
--
作者：
HASEGAWA, T;LECHEVALIER, MP;LECHEVALIER, HA
通讯作者：
LECHEVALIER, HA