Natural infection of small mammal species in Minnesota with the agent of human granulocytic ehrlichiosis
Natural infection of small mammal species in Minnesota with the agent of human granulocytic ehrlichiosis
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DOI：
10.1128/jcm.35.4.853-855.1997
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发表时间：
1997-04-01
影响因子：
9.4
通讯作者：
Dumler, JS
中科院分区：
文献类型：
--
作者：
Walls, JJ;Greig, B;Dumler, JS
人类粒细胞埃利希体病 (HGE) 病原体的天然宿主被怀疑是寄生未成熟阶段肩胛硬蜱的小型哺乳动物，即蜱虫。为了确定此类小型哺乳动物是否自然感染，我们从明尼阿波利斯和明尼苏达州圣保罗的农村和郊区的小型哺乳动物物种中采集了血液和血清样本。样本采集自白足鼠 (Peromyscus leucopus)、东部花栗鼠（Tamias striatus）、南方红背田鼠（Clethrionomysgapperi）和食虫鼩鼱（Blarina brevicauda 和 Sorer cinereus）。通过 PCR 检测血样是否有 HGE 试剂的活动性感染，并通过间接免疫荧光检测白种人小鼠的血清是否有感染的血清学证据。 PCR 分析显示，在测试的 190 个样本中，有 20 个样本 (10.5%) 存在 HGE 剂 DNA。在分析的 119 个白斑鼠血清样本中，12 个样本 (10.1%) 含有马埃利希体抗体。在收集血液和血清的 119 只白斑白鼠中，有 3 只 (2.5%) 存在 HGE 剂 DNA 和抗马大肠杆菌抗体。具有 HGE 剂感染证据的动物广泛分布在明尼阿波利斯-圣路易斯地区。已知有肩胛蜱的区域中的圣保罗地区存在蜱活动，小型哺乳动物是肩胛蜱幼虫的常见宿主，并且在发生 HGE 的地区发现的小型哺乳动物很可能是叮咬人类的受感染蜱的主要宿主。
The natural reservoirs for the agent of human granulocytic ehrlichiosis (HGE) are suspected to be the small mammals that host immature stages of Ixodes scapularis is ticks, To determine if such small mammals are naturally infected, we collected blood and serum samples from small mammal species in rural and suburban areas of Minneapolis and St, Paul, Minn. Samples were collected from white-footed mice (Peromyscus leucopus), eastern chipmunks (Tamias striatus), southern red-backed voles (Clethrionomys gapperi), and insectivorous shrews (Blarina brevicauda and Sorer cinereus). Blood samples were tested by PCR for active infection with the HGE agent, and sera from P. leucopus mice were tested for serologic evidence of infection by indirect immunofluorescence. PCR analyses revealed the presence of HGE agent DNA in 20 of the 190 samples (10.5%) tested, Of the 119 P. leucopus mouse serum samples that were analyzed, 12 (10.1%) contained Ehrlichia equi antibodies. In 3 of 119 (2.5%) P. leucopus mice from which both blood and serum were collected, HGE agent DNA and antibodies against E. equi were present, Animals with evidence of infection,vith the HGE agent are widely distributed around the Minneapolis-St. Paul area in regions with known I. scapularis is tick activity, Small mammals that are frequent hosts for larval I, scapularis ticks and that are found in areas where HGE occurs are likely to be a major reservoir from which infected ticks that bite humans are derived.