Increased B cell-activating factor (BAFF) level in the sputum of children with asthma.
Increased B cell-activating factor (BAFF) level in the sputum of children with asthma.
复制标题
DOI：
10.3345/kjp.2010.53.8.795
复制
发表时间：
2010-08
影响因子：
--
通讯作者：
Kim KE
中科院分区：
文献类型：
--
作者：
Jee HM;Choi BS;Kim KW;Sohn MH;Han MY;Kim KE
B细胞活化因子（BAFF）是肿瘤坏死因子（TNF）超家族成员，以其在B细胞的存活和成熟中的作用而闻名。在幼稚细胞以及效应/记忆T细胞中观察到BAFF活性。我们的目的是探讨痰中BAFF是否在哮喘气道中表达水平升高，并与儿童嗜酸性粒细胞炎症、肺功能和支气管高反应性相关。154名哮喘儿童和98名健康儿童参加了这项研究。收集痰液上清液，测定痰液BAFF和嗜酸性粒细胞阳离子蛋白（ECP）水平。我们进行肺功能测试和乙酰甲胆碱激发试验，同时测量所有受试者的总嗜酸性粒细胞计数，血清总IgE和血清ECP。哮喘儿童诱导痰中BAFF水平[26.50（10.50-100.27）pg/mL]显著高于健康儿童[18.32（7.68-44.63）pg/mL; P=0.011]。痰BAFF与痰嗜酸粒细胞（γ=0.406，P<0.001）和ECP（γ=0.789，P<0.001）呈正相关。痰液BAFF与FEV 1（γ=-0.291，P<0.001）和使用支气管扩张剂后FEV 1（γ=-0.334，P<0.001）呈显著负相关，而与支气管高反应性、血清嗜酸性粒细胞计数和血清ECP无显著相关性。这些发现表明BAFF可能在儿童哮喘中发挥作用，痰中BAFF水平可能是代表气道炎症，特别是嗜酸性粒细胞炎症的支持性标志物。
B cell-activating factor (BAFF) is a tumor-necrosis factor (TNF) superfamily member best known for its role in the survival and maturation of B cells. BAFF activity is observed in naïve cells as well as in effector/memory T cells. We aimed to explore whether BAFF in sputum is expressed at elevated levels in asthmatic airways and associated with eosinophilic inflammation, pulmonary function, and bronchial hyperresponsiveness in children. One hundred and fifty-four asthmatic children and 98 healthy children were enrolled in the study. Sputum supernatants were collected and sputum BAFF and eosinophil cationic protein (ECP) levels were measured. We performed pulmonary function tests and methacholine challenge tests, while measuring total eosinophil count, total serum IgE, and serum ECP in all subjects. Asthmatic children had significantly higher levels of BAFF in induced sputum [26.50 (10.50-100.27) pg/mL] compared to healthy children [18.32 (7.68-44.63) pg/mL; P=0.011]. Sputum BAFF positively correlated with sputum eosinophils (γ=0.406, P<0.001) and sputum ECP (γ=0.789, P<0.001). Significant negative correlations were found between sputum BAFF and FEV1 (γ=-0.291, P<0.001) or post-bronchodilator FEV1 (γ=-0.334, P<0.001), whereas nonsignificant correlations were found between sputum BAFF and bronchial hyperresponsiveness, serum eosinophil count, and serum ECP. These findings suggest that BAFF may play a role in childhood asthma, and BAFF levels in sputum could be a supportive marker that represents airway inflammation, especially eosinophilic inflammation.