Characterization of a honey bee Toll related receptor gene Am18w and its potential involvement in antimicrobial immune defense
Characterization of a honey bee Toll related receptor gene Am18w and its potential involvement in antimicrobial immune defense
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DOI：
10.1051/apido:2004062
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发表时间：
2005-01-01
期刊：
影响因子：
2.4
通讯作者：
Saldivar, E
中科院分区：
文献类型：
--
作者：
Aronstein, K;Saldivar, E
Toll 受体参与细胞内信号转导和昆虫抗菌免疫反应的启动。在这里，我们报告了从蜜蜂蜜蜂中分离和表征的一个新基因（Am18w），该基因编码 Toll 样受体，与家蚕 18 轮虫有惊人的 51.4% 相似性，与果蝇 Toll-7 受体有 46.6% 相似性，与果蝇 18 轮虫有 42.5% 相似性。推导的 18W 蛋白的序列分析揭示了 Toll 和哺乳动物白细胞介素 1 (IL-1) 受体中发现的信号转导受体特征的保守 Toll-白细胞介素 - 抗性信号结构域 (TIR)。在免疫攻击之前和之后的所有发育阶段都检查了蜜蜂 Toll 样受体基因 Am18w 的表达谱。在这里，我们证明，将 dsRNA 探针注射到蜜蜂幼虫中，可以成功破坏目标基因的内源 mRNA，并使我们能够检查 Am18w 基因沉默 (RNAi) 对抗菌肽表达水平的影响。
Toll receptors are involved in intracellular signal transduction and initiation of insect antimicrobial immune responses. Here we report the isolation and characterization of a novel gene (Am18w) from honey bee Apis mellifera, which encodes for the Toll-like receptor and shares a striking 51.4% similarity with Bombyx mori 18-wheeler, 46.6% with Drosophila Toll-7 receptor and 42.5% with Drosophila 18-wheeler. The sequence analysis of the deduced 18W protein revealed a conserved Toll-Interleukin - Resistance signaling domain (TIR) characteristic of signal transducing receptors found in Toll and mammalian interleukin 1 (IL-1) receptors. The expression profiles of the honey bee Toll-like receptor gene Am18w were examined in all developmental stages, before and after immune challenge. Here we demonstrate that injection of a dsRNA probe into honey bee larvae successfully disrupts the endogenous mRNA of the target gene and allows us to examine the effects of Am18w gene silencing (RNAi) on the expression level of antimicrobial peptides.