Microarray analysis of PDGFRα+ populations in ES cell differentiation culture identifies genes involved in differentiation of mesoderm and mesenchyme including ARID3b that is essential for development of embryonic mesenchymal cells
Microarray analysis of PDGFRα+ populations in ES cell differentiation culture identifies genes involved in differentiation of mesoderm and mesenchyme including ARID3b that is essential for development of embryonic mesenchymal cells
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DOI：
10.1016/j.ydbio.2005.12.016
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发表时间：
2006-05-01
影响因子：
2.7
通讯作者：
Nishikawa, SI
中科院分区：
文献类型：
--
作者：
Takebe, A;Era, T;Nishikawa, SI
在早期小鼠胚胎上使用DNA微阵列技术的一个固有困难是其相对较小的尺寸。在这项研究中，我们调查了是否使用ES细胞分化培养，它没有理论上的限制，可以产生的细胞数量，可以改善这种情况。通过DNA微阵列分析了7个不同的ES细胞衍生群体，并检查了其分布模式与PDGFR α（一种涉及中胚层/间充质谱系分化的基因）相似的基因。使用我们实验室开发的软件，我们形成了一组与PDGFR α具有最高相似性的30个基因，其中18个基因被证明参与中胚层、间充质或神经嵴细胞的发育。该列表还包含了几个基因，其在胚胎发生中的作用尚未完全确定。其中一种分子是mARID 3b。mARID 3b表达于中轴中胚层和颅间充质中。mARID 3b缺失小鼠表现出早期胚胎致死性，并且该突变体的大多数表型似乎是由于未能产生足够数量的颅间充质细胞而形成的。这些结果表明，潜在的使用ES细胞分化培养在确定新的基因在胚胎发生中发挥不可或缺的作用。(c)2005年爱思唯尔公司All rights reserved.
An inherent difficulty in using DNA microarray technology on the early mouse embryo is its relatively small size. In this study, we investigated whether use of ES cell differentiation culture, which has no theoretical limit in the number of cells that can be generated, can improve this situation. Seven distinct ES-cell-derived populations were analyzed by DNA microarray and examined for genes whose distribution patterns are similar to those of PDGFR alpha, a gene implicated in differentiation of mesoderm/mesenchymal lineages. Using software developed in our laboratory, we formed a group of 30 genes which showed the highest similarity to PDGFR alpha, 18 of these genes were shown to be involved in development of either mesodermal, mesenchymal or neural crest cells. This list also contains several genes whose role in embryogenesis has not yet been fully identified. One such Molecule is mARID3b. The mARID3b expression is found in the pat-axial mesoderm and cranial mesenchyme. mARID3b-null mouse showed early embryonic lethality, and most phenotypes of this mutant appear to develop from a failure to generate a sufficient number of cranial mesenchymal cells. These results demonstrate the potential use of ES cell differentiation culture in identifying novel genes playing an indispensable role in embryogenesis. (c) 2005 Elsevier Inc. All rights reserved.